Morphology, Stains & Cultures
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Morphology: Pairs | Diplo
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Morphology: Chains | Strepto
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Morphology: Cubical Packet of 4 | Tetrad
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Morphology: Grapelike Clusters | Staphlo
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Morphology: Cubical Packets of 8 | Sarcina
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Morphology: Singles or no specific arrangement | Random
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Morphology: Side-by Side | Palisading
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Morphology: V shaped | Snapping
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Morphology: Oval Shaped | Coccobacillus
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Morphology: One-half Spiral Turn | Vibrio
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Morphology: Loosely Wound Spiral | Spirillum
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Morphology: Tightly Wound Spiral, Corkscrew like | Spirochete
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Morphology: Spherical Shaped | Cocci
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Morphology: Cylindrical or Rod Shaped | Bacilli
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Bacillus Anthracis - Gram Type | Gram Positive, Purple
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Bacillus Anthracis - Morphology | Strepto, bacillus
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Bacillus Anthracis | Endospore forming bacterium and is the causative agent of anthrax.
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Escherichia Coli - Gram Type | Gram Negative, Pink
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Escherichia Coli - Morphology | Random Bacillus
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Streptococcus - Gram Type | Gram Positive, Purple
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Streptococcus - Morphology | Strepto Coccus
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Staphylococcus - Gram Type | Gram Positive - Purple
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Staphylococcus - Morphology | Staphylo Coccus
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Staphylococcus | This is the genus but we used Staphylococcus epidermidis in class several times
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Morphology: Spherical Bacteria in chains | Streptococcus
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Morphology: Spherical Bacteria in pairs | Diplococcus
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Morphology: Rod Shaped Bacteria in chains | Streptobacillus
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Morphology: Rod Shaped Bacteria in pairs | Diplobacillus
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Morphology: Spherical Bacteria in Grape-like Clusters | Staphylococcus
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Tiny Rods which look like cocci | Coccobacillus
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One-half Spiral Turns | Vibrio
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The mixture that provides micro-organisms with the suitable nutrients in the right composition? | Culture Medium
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The liquid culture medium used in class? | Broth
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The solid culture medium used in class? | Agar
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Certain practices that keep the outside organisms from contaminating transferred cultures? | Aseptic Technique
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Normal Incubation Temperature? | 36 C
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Why do we Flame the lip of the tube? | The flaming directs air flow upwards and away from the open tube thereby minimizing the chance of contamination.
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What is the point of a streak plate? | To isolate pure cultures.
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Why is it important to cool a loop after flaming prior to touching it to a culture of microorganisms? | As to not kill the microorganisms
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What is nosocomial? | An infection whose development is favoured by a hospital environment, such as one acquired by a patient during a hospital visit or one developing among hospital staff.
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What is colony morphology? | The features of a particular species which aid in their identification.
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What are agar slants? | Tubed media which is allowed to solidify while slanted, thus allowing for a greater surface area.
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Colony Morphology: Size | Could be described as pinpoint, small, medium, and large.
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Colony Morphology: Elevation | Could be described as flat, raised, convex or umbonate.
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Colony Morphology: Edge | Could be described as entire, undulate, erose, rhizoid, or spreading.
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Colony Morphology: Color | any and all colors could apply
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Colony Morphology: Surface | Could be described as smooth, rough, granular, glistening, wrinkled, or dry.
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Colony Morphology: Odor | Can be useful in identifying the microorganism.
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Odor of Pseudomonas | Sweet, grape-like odor
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Odor of Proteus | Burnt chocolate odor
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Odor of Clostridium | Fecal, putrid odor (this genus as well as other anaerobes)
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Nutrient Agar | This is recommended as a general culture medium for the cultivation of many less fastidious microorganisms as well as a base to which a variety of materials are added to give selective, differential or enriched media.
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Fastidious | organisms that require specialized environments due to complex nutritional requirements
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