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Biology Lab
Morphology, Stains & Cultures
| Question | Answer |
|---|---|
| Morphology: Pairs | Diplo |
| Morphology: Chains | Strepto |
| Morphology: Cubical Packet of 4 | Tetrad |
| Morphology: Grapelike Clusters | Staphlo |
| Morphology: Cubical Packets of 8 | Sarcina |
| Morphology: Singles or no specific arrangement | Random |
| Morphology: Side-by Side | Palisading |
| Morphology: V shaped | Snapping |
| Morphology: Oval Shaped | Coccobacillus |
| Morphology: One-half Spiral Turn | Vibrio |
| Morphology: Loosely Wound Spiral | Spirillum |
| Morphology: Tightly Wound Spiral, Corkscrew like | Spirochete |
| Morphology: Spherical Shaped | Cocci |
| Morphology: Cylindrical or Rod Shaped | Bacilli |
| Bacillus Anthracis - Gram Type | Gram Positive, Purple |
| Bacillus Anthracis - Morphology | Strepto, bacillus |
| Bacillus Anthracis | Endospore forming bacterium and is the causative agent of anthrax. |
| Escherichia Coli - Gram Type | Gram Negative, Pink |
| Escherichia Coli - Morphology | Random Bacillus |
| Streptococcus - Gram Type | Gram Positive, Purple |
| Streptococcus - Morphology | Strepto Coccus |
| Staphylococcus - Gram Type | Gram Positive - Purple |
| Staphylococcus - Morphology | Staphylo Coccus |
| Staphylococcus | This is the genus but we used Staphylococcus epidermidis in class several times |
| Morphology: Spherical Bacteria in chains | Streptococcus |
| Morphology: Spherical Bacteria in pairs | Diplococcus |
| Morphology: Rod Shaped Bacteria in chains | Streptobacillus |
| Morphology: Rod Shaped Bacteria in pairs | Diplobacillus |
| Morphology: Spherical Bacteria in Grape-like Clusters | Staphylococcus |
| Tiny Rods which look like cocci | Coccobacillus |
| One-half Spiral Turns | Vibrio |
| The mixture that provides micro-organisms with the suitable nutrients in the right composition? | Culture Medium |
| The liquid culture medium used in class? | Broth |
| The solid culture medium used in class? | Agar |
| Certain practices that keep the outside organisms from contaminating transferred cultures? | Aseptic Technique |
| Normal Incubation Temperature? | 36 C |
| Why do we Flame the lip of the tube? | The flaming directs air flow upwards and away from the open tube thereby minimizing the chance of contamination. |
| What is the point of a streak plate? | To isolate pure cultures. |
| Why is it important to cool a loop after flaming prior to touching it to a culture of microorganisms? | As to not kill the microorganisms |
| What is nosocomial? | An infection whose development is favoured by a hospital environment, such as one acquired by a patient during a hospital visit or one developing among hospital staff. |
| What is colony morphology? | The features of a particular species which aid in their identification. |
| What are agar slants? | Tubed media which is allowed to solidify while slanted, thus allowing for a greater surface area. |
| Colony Morphology: Size | Could be described as pinpoint, small, medium, and large. |
| Colony Morphology: Elevation | Could be described as flat, raised, convex or umbonate. |
| Colony Morphology: Edge | Could be described as entire, undulate, erose, rhizoid, or spreading. |
| Colony Morphology: Color | any and all colors could apply |
| Colony Morphology: Surface | Could be described as smooth, rough, granular, glistening, wrinkled, or dry. |
| Colony Morphology: Odor | Can be useful in identifying the microorganism. |
| Odor of Pseudomonas | Sweet, grape-like odor |
| Odor of Proteus | Burnt chocolate odor |
| Odor of Clostridium | Fecal, putrid odor (this genus as well as other anaerobes) |
| Nutrient Agar | This is recommended as a general culture medium for the cultivation of many less fastidious microorganisms as well as a base to which a variety of materials are added to give selective, differential or enriched media. |
| Fastidious | organisms that require specialized environments due to complex nutritional requirements |
Created by:
sbiosweitz
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