Exam 2: Recombinant DNA and Biotechnology
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| the production of identical copies through some asexual means | cloning
🗑
|
||||
| examples of clones | bacteria in a colony all come from the same original cell and therefore are clones; human identical twins
🗑
|
||||
| sections of DNA that express a characteristic | genes
🗑
|
||||
| the production of many identical copies of the same gene | gene cloning
🗑
|
||||
| DNA put together from 2 or more different sources | recombinant
🗑
|
||||
| (restriction endonucleases) produced by cells to _____ any _____ in order to protect the organism | chew up any foreign DNA
🗑
|
||||
| (restriction endonucleases) many recognize and _____ specific sequences | cut at
🗑
|
||||
| (restriction endonucleases) most do not cut the double strand _____, they... | evenly; leave ends that overhang by 4 to 8 bases (sticky ends)
🗑
|
||||
| 2 DNA fragments that have been cut with the same enzyme can be... | stuck together
🗑
|
||||
| an enzyme called _____ can repair the break in the backbone joining them permanently | DNA ligase
🗑
|
||||
| many organisms in addition to their main chromosomes have small, circular bits of DNA called _____ | plasmids
🗑
|
||||
| plasmids usually only carry only a few _____ | genes
🗑
|
||||
| plasmids replicate _____ of the main chromosomes | independently
🗑
|
||||
| cells can have from _____ to ______ copies of plasmids | one to several thousand
🗑
|
||||
| plasmids can be used to produce _____ in bacteria or yeast (cloning) | foreign DNA
🗑
|
||||
| recombinant DNA technology: cut target DNA with ... | restriction endonuclease
🗑
|
||||
| recombinant DNA technology: cut plasmid DNA with... | the same endonuclease
🗑
|
||||
| bond complementary sticky ends | anneal
🗑
|
||||
| attach permanently | ligate
🗑
|
||||
| transfer the recombinant plasmid into... | an E. coli or yeast
🗑
|
||||
| E. coli | prokaryote
🗑
|
||||
| yeast | eukaryote
🗑
|
||||
| as the E. coli or yeast reproduce, it produces... | more genes or more of a product like insulin
🗑
|
||||
| PCR | Polymerase Chain Reaction
🗑
|
||||
| PCR: creates copies of DNA _____ in a test tube | very quickly
🗑
|
||||
| PCR: makes lots of copies of... which can be less than one part per million of the total DNA sample | targeted sequence of DNA
🗑
|
||||
| PCR: uses _____ (the enzyme that carries out DNA replication) to cause a chain reaction that continues until the process is stopped | DNA polymerase
🗑
|
||||
| (analyzing DNA segments) 1st PCR is used to _____ the amount of DNA that we are interested in | increase
🗑
|
||||
| (analyzing DNA segments) then the DNA is treated with _____ | restricted endonucleases (this creates a unique collection of different-sized fragments)
🗑
|
||||
| (analyzing DNA segments) the DNA is then placed into a process called _____ | gel electrophorosis
🗑
|
||||
| (gel electrophorosis) a gel of _____ with wells in the top is poured | agarose
🗑
|
||||
| (gel electrophorosis) DNA that has been cut with _____ is placed into the wells | endonucleases
🗑
|
||||
| (gel electrophorosis) an electric current is run across the gel with the _____ charge at the bottom | positive
🗑
|
||||
| (gel electrophorosis)the negatively charged DNA fragments move toward the _____ charge | positive
🗑
|
||||
| (gel electrophorosis) smaller fragments move _____; larger fragments move _____ | (small) faster; (large) more slowly
🗑
|
||||
| (gel electrophorosis) the sample forms a _____ of DNA fragments of increasing size | "ladder"
🗑
|
||||
| DNA is collected at... | the crime scene and from crime suspects
🗑
|
||||
| DNA is exposed to _____ and put into _____ | restriction enzymes; gel electrophoresis
🗑
|
||||
| different people have... | different sequences of DNA
🗑
|
||||
| different sequences means different... | "ladders" will show up in the electrophoresis
🗑
|
||||
| if a suspect's DNA "ladder" matches the crime scene DNA ladder, then... | the 2 DNA samples come from the same person
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
chelsea_2010
Popular Biology sets