Some microscope, and Nuclear & cytoplasmic staining (trouble shooting too)
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| What are the objective lens systems | Scanning , intermediate, high power and oil immersion
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| what is determined by multiplying the magnifications of the oculare and the objective lens | total magnification
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| what is the resolving power | the ability to reveal fine detail or to discriminate between adjacent details
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| Define Chromatic aberration | white light enters a lens and is refracted into the colors of the visible light spectrum
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| what is the difference between Achromatic and Apchromatic | achromatic is corrected for two collors while apochromatic is corrected for three colors
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| all objectives will have the focal point in the same plane | parafocality
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| what are 3 kinds of polarizing microscopes | birefringence, anistropism, double refraction
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| produces 3d images | scanning electron microscope
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| where tissue is penetrated by or absorbs a dye solution and become colored without any other change or chemical reaction occuring | absorption
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| accumulation of stain by surface of a tissue component | adsorption
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| when dye and substance to be dyed develop diffferent charges | Ionic
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| atoms share electron | covalent bonding
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| stains applied to living tissue | vital stains
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| anionic | a negative charge dye or tissue component (acidic)
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| a positive charge dye or tissue component | basic/cationic
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| basophillic | an acidic substnce easily stainable with basic dye
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| a basic substance easily stainable with acidic dye | acidophilic
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| a protein may be positve or negatively charge, depening on ph of solution | amphoteric
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| point where positive and negative charges are equal | Isoelectrical point (IEP)
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| Eosin pH | 4.6 to 5
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| below 6 | net charge is positive (acidophillic)
Attraction of anionic dyes (negative)
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| above 6 | net charge is negative (basophilic)
attraction of cationic dye(positive)
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| what happens if eosin ph drops too low | coo groups recombines with hydrogen
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| what are dyes | organic compound(coal tar, benzene derivative)
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| the group that confers the property of color | Chromophore
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| Common property of chormophores | easily reduce due to affinity for hydrogen
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| Define chromagen | a benzene derivative containing chormophoric groups
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| an ionizing group required to able dye to link firmly to tissue | auxochrome
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| give examples of chromophores and auxochrome | trinitrobenzene & picric acid
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| cationic or anionic depending on Ph & IEP | amphoteric
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| four Natural dyes | Carmine,orcein,saffron, hematoxylin
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| factors affecting dye binding | PH, Temperature,Concentration,Salts, Fixatives
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| tissue that reacts primarily w/the carboxyl(-cooh) and Hydroxyl(-oh) groups | potassium dichromate
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| Mordant | link between dye and tissue
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| mordant to dye | Lake
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| 3 methods of differentiating sections when mordant dyes are used regressively | basic or cationic dyes are differentiated by week acid solution
-excess moredant will break the tissue
-oxidizing the dye to a colorless substance
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| true or false: aluminum hematoxylin is reistant to decolorization with acid | false
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| name two oxidizing differentiators | potassium permanganate & chromium trioxide
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| Hematin | it is a pigment
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| Hematein | Oxidized hematoxylin
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| Ripening= | oxidation
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| when is aluminum sulfate used | to prepare gill hematoxylin
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