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H&E stains

Some microscope, and Nuclear & cytoplasmic staining (trouble shooting too)

QuestionAnswer
What are the objective lens systems Scanning , intermediate, high power and oil immersion
what is determined by multiplying the magnifications of the oculare and the objective lens total magnification
what is the resolving power the ability to reveal fine detail or to discriminate between adjacent details
Define Chromatic aberration white light enters a lens and is refracted into the colors of the visible light spectrum
what is the difference between Achromatic and Apchromatic achromatic is corrected for two collors while apochromatic is corrected for three colors
all objectives will have the focal point in the same plane parafocality
what are 3 kinds of polarizing microscopes birefringence, anistropism, double refraction
produces 3d images scanning electron microscope
where tissue is penetrated by or absorbs a dye solution and become colored without any other change or chemical reaction occuring absorption
accumulation of stain by surface of a tissue component adsorption
when dye and substance to be dyed develop diffferent charges Ionic
atoms share electron covalent bonding
stains applied to living tissue vital stains
anionic a negative charge dye or tissue component (acidic)
a positive charge dye or tissue component basic/cationic
basophillic an acidic substnce easily stainable with basic dye
a basic substance easily stainable with acidic dye acidophilic
a protein may be positve or negatively charge, depening on ph of solution amphoteric
point where positive and negative charges are equal Isoelectrical point (IEP)
Eosin pH 4.6 to 5
below 6 net charge is positive (acidophillic) Attraction of anionic dyes (negative)
above 6 net charge is negative (basophilic) attraction of cationic dye(positive)
what happens if eosin ph drops too low coo groups recombines with hydrogen
what are dyes organic compound(coal tar, benzene derivative)
the group that confers the property of color Chromophore
Common property of chormophores easily reduce due to affinity for hydrogen
Define chromagen a benzene derivative containing chormophoric groups
an ionizing group required to able dye to link firmly to tissue auxochrome
give examples of chromophores and auxochrome trinitrobenzene & picric acid
cationic or anionic depending on Ph & IEP amphoteric
four Natural dyes Carmine,orcein,saffron, hematoxylin
factors affecting dye binding PH, Temperature,Concentration,Salts, Fixatives
tissue that reacts primarily w/the carboxyl(-cooh) and Hydroxyl(-oh) groups potassium dichromate
Mordant link between dye and tissue
mordant to dye Lake
3 methods of differentiating sections when mordant dyes are used regressively basic or cationic dyes are differentiated by week acid solution -excess moredant will break the tissue -oxidizing the dye to a colorless substance
true or false: aluminum hematoxylin is reistant to decolorization with acid false
name two oxidizing differentiators potassium permanganate & chromium trioxide
Hematin it is a pigment
Hematein Oxidized hematoxylin
Ripening= oxidation
when is aluminum sulfate used to prepare gill hematoxylin
Created by: cristalberrios
 

 



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