biochemical tests 1-9
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| What are hydrolysis reactions? | they require hydrolases and water
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| What is the substrate and enzyme for gelatin hydrolysis? | Gelatin and gelatinase
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| What is the End Product of Gelatin Hydrolysis? | Amino Acids
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| What is the incubation temp and time for Gelatin Hydrolysis? | 25 degrees and 48 hrs
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| What is the indicator for Gelatin Hydrolysis? | there is none
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| What is the substrate and enzyme for Starch Hydrolysis? | Starch and Amylase
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| What is the indicator for Starch Hydrolysis | Iodine
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| What is the end product of Starch Hydrolysis? | Glucose
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| What is the incubation temp and time for Starch Hydrolyis? | 37 degrees and 24-48hrs
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| What is Casein Hydrolysis? | Protein Hydrolysis or Proteolysis
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| What is the substrate and enzyme in Casein hydrolysis? | Casein and caseinase
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| What is the indicator in Casein Hydrolysis? | there is none
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| What is the end product of Casein Hydrolysis ? | amino acids
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| What is the incubation temp and time of Casein Hyrdolysis? | 37 degrees and 24-48 hrs
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| What is the medium used in Lipid/Fat Hydrolysis? | Spirit blue agar
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| What is the substrate and enzyme used in Lipid/Fat Hydrolysis? | Triglyceride and Lipase
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| What is the indicator in Lipid/Fat hydrolysis? | Spirit Blue indicator
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| What is the end product of Lipid/Fat hydrolysis? | Fatty acids and glycerol
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| What is the temp and incubation time of Lipid/Fat Hydrolysis? | 37 degrees and 24-48 hrs
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| What are some non fermenting bacteria? | Pseudomonas aeruginosa, Pseudomonas fluorescens, and Alcaligenes faecalis
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| What parts are used in a Phenol Red Test? | Sugar substrate, Phenol Red Indicator, Inverted vial (gas), and Protein
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| What sugars are used in a Phenol Red Test? | glucose, lactose, and sucrose
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| What is the starting pH of Phenol Red Test? | 7.4
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| What color is a positive Phenol Red Test? | yellow and pH less than 7
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| What color is a negative Phenol Red Test? | red and pH more than 7
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| What is the substrate in Litmus Milk Test? | Casein, Lactose, and Litmus Blue indicator
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| What are the enzymes in Litmus Milk test? | Lactase or Beta (B) and Caseinase
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| What is the indicator in Litmus Milk test? | Litmus blue
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| What are the acidic results of a Litmus Milk test? | Acidic pH less than or equal to 4.5 and pink color
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| What are the basic/alkaline results of a Litmus milk test? | pH above or equal to 8.3 and a purple color
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| End products of Litmus Milk test? | Acids, Alcohols, ammonia, soft curd or hard curd formation
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| Litmus blue indicator turns what color with ammonia production? | purple
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| What enzyme secreted by bacteria result in a hard clot formation? | Rennin
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| what is Peptonization? | dissolving of clot by proteolytic enzymes, tube becomes liquid and turns brownish-purple
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| What is stormy fermentation? | Anaerobic gas fermentation in a hard clot at the bottom of tube
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| Litmus blue reduction? | bacteria reduce the litmus blue indicator to a white color
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