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Microbio ch13

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Question
Answer
Define: fomite, (do not have to know specifics)   show
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BSL   show
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show Destruction of all microbial life (includes viruses)  
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disinfection   show
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show Lower microbial counts to safe levels/ usually in commercial establishments/ This is done by high temperature washing & dipping in a disinfectant  
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show Removing microbes from living tissue (antiseptics used for this, usually with chemicals)  
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Understand antisepsis and DEGERMING.   show
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show Used inside the body or penetrate tissues/blood// Must be sterile  
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show Contact mucous membranes of unbroken skin/ High level disinfection, no sterilization  
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What are non-critical items and which protocol is used for each?   show
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Understand the four factors to consider when choosing a method of controlling microbial growth.   show
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show Kills by oxidation effects: flaming, incineration, hot-air sterilization  
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Understand heat methods, including, moist heat/autoclaves   show
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show Eliminates pathogens and lower microbial numbers to prolong life of product/ Ex: Classical 63ºC,30min//HTST 72ºC,17min//UHT Sterilizes milk  
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show Effect how temps are depending on kind of bacteria or microbe/ Psychrophiles continue to grow/ Bacteria may go dormant/ Some bacteria preserved by freezing, others killed (EX: Eukaryotic)  
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Understand pressure   show
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show Ionizing & non-ionizing radiation  
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show HEPA filters remove microbes, endospores, and viruses Membrane filtration removes bacteria from liquids (that cannot be autoclaved)  
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Know the examples of chemical methods of microbial control: phenols and phenolics, pt1   show
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show Cresols: from coal tar, in Lysol/ Bisphenols: Hexachlorophene is good for Gram + bacteria & Triclosan- in soaps, toothpastes, sheets, etc. Good against Gram + and - bacteria  
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show Oligodynamic action-Very small amounts exert antimicrobial activity/ Denature proteins/ AG,Hg,Cu,Zn  
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Know the examples of chemical methods of microbial control: heavy metals, pt2   show
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show Tincture: solution in aqueous alcohol/ Iodophor: combines with organic molecules(betadine)/ Oxidizes cellular components  
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Know the examples of chemical methods of microbial control: chlorine   show
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show Most common: ethanol, isopropanol/ Disrupts membranes/ kill bacteria and fungi, but not endospores or non-enveloped viruses/ Degerming  
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Know the examples of chemical methods of microbial control: surfactants   show
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Know the examples of chemical methods of microbial control: quats   show
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Know the examples of chemical methods of microbial control: bisbiguanides   show
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Know the examples of chemical methods of microbial control: alkylating agents   show
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Know the examples of chemical methods of microbial control: peroxygens   show
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show Compressed state/ CO2 at 10x atmospheric pressure/ Sterilizes/ Enters cell, forms carbonic acid, lowers pH (Works with vegetative cells & endospores together with peracetic acid)/ Food, medical devices, tissues  
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show spread a place with bacteria/ place filter disks soaked in diff disinfectants on top/ incubate/ determine lawn grows/ extent  
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show Provides a minimum inhibitory concentration (MIC)  
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Understand the disk-diffusion method, e-test, use-dilution test, and in-use test.   show
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Understand the disk-diffusion method, e-test, use-dilution test, and in-use test.   show
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show Solute concentration is higher than that inside the cell. Water flows out of cell and into the surrounding solution  
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Created by: Clinton Perdue
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