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Micro-Lab Pr. 1

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Question
Answer
show 1-Flood w/primary stain (crystal violet) for 1-min, rinse w/H2O. 2-Flood w/mordant (iodine) for 1-min, rinse w/H2O. 3-Rinse w/decolorizer (1/2 alcohol, 1/2 acetone) for 3-5 secs, rinse w/H2O. 4-Flood w/counterstain (safranin) for 1 min, rinse w/H2O, blot.  
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Why do you heat fix slides?   show
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microbe is put in or on a ____   show
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show culture  
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show inoculation  
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moving microbe to media without contamination   show
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steps of aseptic transfer   show
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rules of care for microscope   show
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Endospore stain procedure   show
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alpha hemolysis   show
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show complete hemolysis; clear halos  
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show non-hemolytic; no destruction to RBCs  
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Acid Fast stain steps   show
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acid fast stain result fuschia   show
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acid fast stain result blue   show
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Acid fast stain: what does primary stain (carbolfuchsin) break down?   show
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show differential stain; protective sugar coating  
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show background; simple  
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acid fast is used to stain what bacteria?   show
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show tuberculosis and leprosy  
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fermentation test: red result?   show
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show homolactic  
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show heterolactic  
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show fermentation happening and pH is acidic  
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endospore stain: what stains green? what stains pink?   show
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Eukarya slides: protista   show
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Eukarya slides: fungus   show
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show carbolfuchsin, acid-alcohol, methylene blue  
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show crystal violet, iodine, 1/2 alcohol 1/2 acetone, safrinin  
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show malachite green, water, safrinin  
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two bacteria that can produce endospores for survival?   show
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Created by: nurse savage
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