Micro-Lab Pr. 1
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| Gram stain procedure | 1-Flood w/primary stain (crystal violet) for 1-min, rinse w/H2O. 2-Flood w/mordant (iodine) for 1-min, rinse w/H2O. 3-Rinse w/decolorizer (1/2 alcohol, 1/2 acetone) for 3-5 secs, rinse w/H2O. 4-Flood w/counterstain (safranin) for 1 min, rinse w/H2O, blot.
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| Why do you heat fix slides? | stick (adhere) the microbe to the slide and to kill the microbe
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| microbe is put in or on a ____ | media
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| microbe growing in or on a media | culture
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| putting any microbe in or on a media | inoculation
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| moving microbe to media without contamination | aseptic transfer
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| steps of aseptic transfer | turn on bunsen burner, flame inoculating loop/needle, gently shake to cool, flame microbe tube, get microbe specimen, flame microbe tube, flame media tube, put specimen onto/into media, flame media tube, flame inoculating loop/needle
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| rules of care for microscope | always use 2 hands, start with 4x objective, use coarse focus only with 4x and 10x objectives, lens paper, stage down and slide off, light down and off, 4x objective in place, cord in place
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| Endospore stain procedure | 1-Flood with primary stain (malachite green) with filter paper over steam 15-mins, rinse with H2O. 2-Rinse with decolorizer (H2O). 3-Flood with counterstain (safranin) 1-min, rinse with H2O, blot dry.
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| alpha hemolysis | partial hemolysis; green halos
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| beta hemolysis | complete hemolysis; clear halos
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| gamma hemolysis | non-hemolytic; no destruction to RBCs
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| Acid Fast stain steps | 1-Flood w/primary stain (carbolfuchsin) w/filter paper over steam 15-mins, rinse w/ H2O. 2-Rinse w/decolorizer (acid-alcohol) 5-10 secs, rinse w/H2O. 3-Flood w/counterstain (methylene blue) for 2-mins, rinse w/H2O, blot dry
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| acid fast stain result fuschia | acid fast
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| acid fast stain result blue | non-acid fast
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| Acid fast stain: what does primary stain (carbolfuchsin) break down? | mycolic acid (waxy lipids)
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| Capsule stain: is it simple or differential? What is a capsule? | differential stain; protective sugar coating
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| negative stain: what is being stained? simple or differential stain? | background; simple
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| acid fast is used to stain what bacteria? | mycobacterium
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| two mycobacterium diseases | tuberculosis and leprosy
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| fermentation test: red result? | no fermentation; pH alkaline
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| fermentation test: yellow result with no bubble | homolactic
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| fermentation test: yellow result with bubble | heterolactic
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| fermentation test: turning yellow means what? | fermentation happening and pH is acidic
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| endospore stain: what stains green? what stains pink? | green-endospores, pink-bacteria
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| Eukarya slides: protista | Balantidium
Toxoplasma
Trichomonas
Trypanosoma
Candida
Plasmodium
Giardia
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| Eukarya slides: fungus | Penicillium
Aspergillus
Rhizopus
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| Acid fast: primary stain? decolorizer? counterstain? | carbolfuchsin, acid-alcohol, methylene blue
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| Gram stain: primary stain? mordant? decolorizer? counterstain? | crystal violet, iodine, 1/2 alcohol 1/2 acetone, safrinin
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| Endospore stain: primary stain? decolorizer? counterstain? | malachite green, water, safrinin
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| two bacteria that can produce endospores for survival? | Clostridium botuli, Bacillus anthracis
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