Micro-Lab Pr. 1
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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Gram stain procedure | show 🗑
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Why do you heat fix slides? | show 🗑
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microbe is put in or on a ____ | show 🗑
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microbe growing in or on a media | show 🗑
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show | inoculation
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show | aseptic transfer
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show | turn on bunsen burner, flame inoculating loop/needle, gently shake to cool, flame microbe tube, get microbe specimen, flame microbe tube, flame media tube, put specimen onto/into media, flame media tube, flame inoculating loop/needle
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rules of care for microscope | show 🗑
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show | 1-Flood with primary stain (malachite green) with filter paper over steam 15-mins, rinse with H2O. 2-Rinse with decolorizer (H2O). 3-Flood with counterstain (safranin) 1-min, rinse with H2O, blot dry.
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show | partial hemolysis; green halos
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show | complete hemolysis; clear halos
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gamma hemolysis | show 🗑
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show | 1-Flood w/primary stain (carbolfuchsin) w/filter paper over steam 15-mins, rinse w/ H2O. 2-Rinse w/decolorizer (acid-alcohol) 5-10 secs, rinse w/H2O. 3-Flood w/counterstain (methylene blue) for 2-mins, rinse w/H2O, blot dry
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acid fast stain result fuschia | show 🗑
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acid fast stain result blue | show 🗑
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Acid fast stain: what does primary stain (carbolfuchsin) break down? | show 🗑
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show | differential stain; protective sugar coating
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negative stain: what is being stained? simple or differential stain? | show 🗑
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acid fast is used to stain what bacteria? | show 🗑
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two mycobacterium diseases | show 🗑
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fermentation test: red result? | show 🗑
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show | homolactic
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fermentation test: yellow result with bubble | show 🗑
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show | fermentation happening and pH is acidic
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show | green-endospores, pink-bacteria
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show | Balantidium
Toxoplasma
Trichomonas
Trypanosoma
Candida
Plasmodium
Giardia
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Eukarya slides: fungus | show 🗑
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Acid fast: primary stain? decolorizer? counterstain? | show 🗑
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show | crystal violet, iodine, 1/2 alcohol 1/2 acetone, safrinin
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show | malachite green, water, safrinin
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show | Clostridium botuli, Bacillus anthracis
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Created by:
nurse savage
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