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Micro-Lab Pr. 1

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
Gram stain procedure   show
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Why do you heat fix slides?   show
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microbe is put in or on a ____   show
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microbe growing in or on a media   show
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show inoculation  
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show aseptic transfer  
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show turn on bunsen burner, flame inoculating loop/needle, gently shake to cool, flame microbe tube, get microbe specimen, flame microbe tube, flame media tube, put specimen onto/into media, flame media tube, flame inoculating loop/needle  
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rules of care for microscope   show
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show 1-Flood with primary stain (malachite green) with filter paper over steam 15-mins, rinse with H2O. 2-Rinse with decolorizer (H2O). 3-Flood with counterstain (safranin) 1-min, rinse with H2O, blot dry.  
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show partial hemolysis; green halos  
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show complete hemolysis; clear halos  
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gamma hemolysis   show
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show 1-Flood w/primary stain (carbolfuchsin) w/filter paper over steam 15-mins, rinse w/ H2O. 2-Rinse w/decolorizer (acid-alcohol) 5-10 secs, rinse w/H2O. 3-Flood w/counterstain (methylene blue) for 2-mins, rinse w/H2O, blot dry  
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acid fast stain result fuschia   show
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acid fast stain result blue   show
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Acid fast stain: what does primary stain (carbolfuchsin) break down?   show
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show differential stain; protective sugar coating  
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negative stain: what is being stained? simple or differential stain?   show
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acid fast is used to stain what bacteria?   show
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two mycobacterium diseases   show
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fermentation test: red result?   show
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show homolactic  
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fermentation test: yellow result with bubble   show
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show fermentation happening and pH is acidic  
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show green-endospores, pink-bacteria  
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show Balantidium Toxoplasma Trichomonas Trypanosoma Candida Plasmodium Giardia  
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Eukarya slides: fungus   show
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Acid fast: primary stain? decolorizer? counterstain?   show
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show crystal violet, iodine, 1/2 alcohol 1/2 acetone, safrinin  
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show malachite green, water, safrinin  
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show Clostridium botuli, Bacillus anthracis  
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Created by: nurse savage
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