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CoNS Q/A

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Question
Answer
What are the characteristics of Coagulase Negative Staphylococci (CoNS) when stained with Gram stain?   The CoNS would be characterized as Gram-positive cocci in clusters, meaning they appear round and purple.  
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How would CoNS appear on a Blood Agar Plate in terms of hemolysis and colony?   CoNS is non-hemolytic and grows in white, flat colonies.  
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Which media or testing method can be used to distinguish CoNS from Staphylococcus aureus? What would the CoNS positive look like?   Mannitol Salt Agar; the colonies would appear pink because CoNS cannot ferment mannitol like Staph. aureus.  
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Which two chemical components can differentiate CoNS from Staphylococcus saprophyticus?   Novobiocin (antibiotic) and Trehalose-Mannitol-Phosphatase agar (media)  
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What are some effects of the "slime" virulence factor produced by Coagulase Negative Staphylococci?   -Harder to treat -Inhibits neutrophil chemotaxis and phagocytosis -Inhibits Vancomycin -Enhances adherence to plastic surfaces  
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In which body areas can CoNS be found as normal flora?   Upper respiratory tract, skin, intestinal tract, mucous membranes  
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