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Virus Module Set 3

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Question
Answer
Virus assays:   Used to determine quantity of viruses in a sample  
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Two types of approaches   Count particles and measure concentration of infectious units  
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Direct count:   Made with an electron microscope  
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Indirect count:   Determines highest dilution of virus that causes red blood cells to clump together  
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Plaque assays are   Dilutions of virus preparation made and plated on lawn of host cells  
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Plaque assays measure   Number of plaques counted  
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Plaque assays results are expressed as   PFU (plaque forming units)  
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Virion sizes range from _____ nm on diameter   10-400  
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Most virions must be   Viewed with an electron microscope  
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All virions contain   Nucleocapsid  
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Nucleocapsid is composed of   Nucleic acid (DNA or RNA) and a protein coat (capsid)  
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Viruses also made up of   Envelope  
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Nucleocapsid:   Nucleic acid held within protein coat  
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Capsid:   Protein coat that surrounds viral genome  
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Capsid function   Protects genome Aids in transfer obetween host cells  
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protomer:   protein subunits that make up capsid  
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Created by: Whereis_raye
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