Virus Module Set 3
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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Virus assays: | Used to determine quantity of viruses in a sample
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Two types of approaches | Count particles and measure concentration of infectious units
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Direct count: | Made with an electron microscope
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Indirect count: | Determines highest dilution of virus that causes red blood cells to clump together
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Plaque assays are | Dilutions of virus preparation made and plated on lawn of host cells
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Plaque assays measure | Number of plaques counted
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Plaque assays results are expressed as | PFU (plaque forming units)
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Virion sizes range from _____ nm on diameter | 10-400
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Most virions must be | Viewed with an electron microscope
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All virions contain | Nucleocapsid
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Nucleocapsid is composed of | Nucleic acid (DNA or RNA) and a protein coat (capsid)
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Viruses also made up of | Envelope
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Nucleocapsid: | Nucleic acid held within protein coat
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Capsid: | Protein coat that surrounds viral genome
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Capsid function | Protects genome
Aids in transfer obetween host cells
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protomer: | protein subunits that make up capsid
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