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Virus Module Set 3

Virus assays: Used to determine quantity of viruses in a sample
Two types of approaches Count particles and measure concentration of infectious units
Direct count: Made with an electron microscope
Indirect count: Determines highest dilution of virus that causes red blood cells to clump together
Plaque assays are Dilutions of virus preparation made and plated on lawn of host cells
Plaque assays measure Number of plaques counted
Plaque assays results are expressed as PFU (plaque forming units)
Virion sizes range from _____ nm on diameter 10-400
Most virions must be Viewed with an electron microscope
All virions contain Nucleocapsid
Nucleocapsid is composed of Nucleic acid (DNA or RNA) and a protein coat (capsid)
Viruses also made up of Envelope
Nucleocapsid: Nucleic acid held within protein coat
Capsid: Protein coat that surrounds viral genome
Capsid function Protects genome Aids in transfer obetween host cells
protomer: protein subunits that make up capsid
Created by: Whereis_raye
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