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Bio Chapter 9

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Term
Definition
Restriction enzyme   An enzyme that cuts double-stranded DNA into fragments by recognizing specific nucleotide sequences and cutting the DNA at those sequences  
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Gel electrophoresis   Method of separating various lengths of DNA strands by applying an electrical current to a gel  
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Restriction map   Diagram that shows the lengths of fragments between restriction sites in the strand of DNA  
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Polymerase chain reaction (PCR)   Method for increasing the quantity of DNA by separating it into two strands and adding primers and enzymes  
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DNA fingerprint   A pattern of DNA characteristics that is unique, or nearly so, to an individual organism  
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clone   An organism, cell, or piece of genetic material that is genetically identical to one from which it was derived; to make a genetic duplicate  
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Genetic engineering   A technology in which the genome of a living cell is modified for medical or industrial use  
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Recombinant DNA   DNA molecules that are artificially created by combining DNA from different sources  
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Plasmid   A genetic structure that can replicate independently of the main chromosome(s) of a cell; usually, a circular DNA molecule in bacteria (prokaryotes)  
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Transgenic   Organism whose genome has been altered to contain one or more genes from another organism or species  
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Gene knockout   Genetic manipulation in which one or more of an organism's genes are prevented from being expressed  
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Genomics   The study of entire genomes, especially by using technology to compare genes within and between species  
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Gene sequencing   Process of determining the order of DNA nucleotides in genes and genomes  
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Human Genome Project   A research effort to sequence and locate the entire collection of genes in human cells  
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Bioinformatics   The application of information technologies in biology, especially in genetics  
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DNA microarray   research tool used to study gene expression  
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Proteomics   Study and comparison of all the proteins produced by an organism's genome  
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Genetic screening   Process of testing DNA to determine the chance a person has, or might pass on, a genetic disorder  
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Gene therapy   A technique that places a gene into a cell to correct a hereditary disease or to improve the genome  
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Primer   A short, single-stranded fragment of DNA or RNA that is required for the initiation of DNA replication  
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Created by: mariazierolf
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