EXAM 2
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Allosteric enzyme | An enzyme that has multiple and regulatory active sites.It controls the metabolic pathways through environmental signals, including the final product.Its kinetics are complex
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| Reversible inhibitors | Competive, uncompetitive,and noncompetive
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| Irreversible inhibitors | Group specific reagents,affinity labels,suicide inhibitors, transition state analogs
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| Reversible inhibition characteristics | -rapid dissociation of the enzyme-inhibitor complex
-rapid dissiciation of the inhibitor with the enzyme
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| Competive inhibition | the inhibitor resembles the substrate and binds to the active site of the enzyme.An enzyme can bind substrate forming an ES complex or inhibitor EI.
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| competive unhibition kinetics | increases KM
No change on Vmax
Can be overcome by increasing substrate concentration
Graph is a V with the same Y-intercept
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| Uncompetive inhibition | It is substrate-dependent inhibition in that the inhibitor binds only to the enzyme-substrate complex (ES)
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| uncompetitive inhibition kinetics | lowers Vmax
Lovers KM
Parallel graph, different x and Y intercept
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| Noncompetitive inhibition | The inhibitor and the substrate can bind simultaneously to an enzyme molecule at different binding sites. Decreases the overall number of active enzymes
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| Noncompetitive inhibition kinetics | Lowers apparent Vmax
KM is unchanged
the graph is a V with the same x-intercept and different y-intercept
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| Irreversible inhibitor characteristics | It dissociates very slowly from its target enzyme because it has become tightly bound to the enzyme, either covalently or noncovalently
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| Group-specific reagents | Modify specific R groups of amino acids
Ex: Nerve gas DIPF react with serine residues
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| Affinity labels, also called substrate analogs | They are structurally similar to the substrate, inhibit enzymes by covalently modifying active sites residues
Ex: TPCK reacts with His 57 in chymotrypsin
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| Suicide Inhibitors, or mechanism-based inhibitors | They chemically modify the substrates. The enzyme starts the reaction but the inhibitor forms a reactive intermediate that inactivates the enzyme covalently modifying it.
Ex: Penicillin
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| Transition-state analogs |
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Or sort by any of the columns using the down arrow next to any column heading.
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Created by:
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