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Human Genetics, DNA Structure and Function, Gene Expression, Biotechnology

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Term
Definition
incomplete dominance   no allele is completely dominant, heterozygous shows "blending"  
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codominance   when different alleles are present, phenotypes of both are expressed in their pure form  
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pleiotropy   one gene effects more than one phenotypic characteristics (e.g. Marfan Syndrome, Sickle Cell Anemia  
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Epistasis   expression of gene that may be masked by alleles from second gene  
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polygenic inheritance   phenotype by additive effects of 2 or more genes  
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DNA Polymerase   enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA; important in DNA replication  
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RNA Polymerase   an enzyme that is responsible for making RNA from DNA template  
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mRNA   A minute particle consisting of RNA and associated proteins, found in large numbers in the cytoplasm of living cells. They bind messenger RNA and transfer RNA to synthesize polypeptides and proteins  
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tRNA   small RNA molecules that carry amino acids to the ribosome for polymerization into the growing polypeptide chain when the anticodon of the tRNA pairs with a codon on the mRNA being translated  
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rRNA   a molecular component of a ribosome, the cells essential protein factory. Strictly speaking, it does not make proteins. It makes polypeptides that go to make up the proteins  
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introns   a segment of a DNA or RNA molecule that does not code for proteins and interrupts the sequence of genes  
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exons   a segment of a DNA or RNA molecule that together containing information coding for a protein or peptide sequence  
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transcription   nucleus; mRNA made by enzyme RNA Polymerase; RNA splicing; introns removed; exons left in; used as instruction for making protein  
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translation   cytoplasm-on ribosomes made of protein and mRNA; synthesis of protein using mRNA as instructions; must get proper order if amino acids; start/stop codons; requires tRNA  
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protein   end product of translation. It is made  
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silent mutation   changing 3rd nucleotide may change amino acid due to redundancy in the triplet code  
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missense mutation   changes one amino acid (usually from change in single nucleotide)  
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nonsense mutation   get stop codon instead of amino acid and translation stops too soon  
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frameshift   a deletion or insertion of nucleotides can change every amino acid that follows. Usually results in nonfunctional protein  
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progeria   premature aging disorde; nucleotide 1824 changed from C to T, mRNA and protein is incomplete  
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stem cells   undifferentiated biological cells that can differentiate into specialized cells and can divide, through mitosis, to produce more stem cells  
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restriction enzymes   cut up foreign DNA, into fragments at specific locations, that gets into cell  
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Polymerase Chain Reaction   makes millions of copies of DNA, then sent for sequencing, visualized by gel electrophoresis, or cloned into a plasmid for further experiments  
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gene cloning   making copies of genes using bacteria  
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therapeutic cloning   obtain embryonic stem cells that are a genetic match to patient; combine donor egg with cell from patient  
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reproductive cloning   been done in sheep, horses, cattle, mice, cats, etc., against law for humans  
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transgenic organisms   an organism that expresses DNA from another species through recombinant technology. Can be single cell or multicellular.  
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gene therapy    
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