Lab 5
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Formed from megakaryocytes residing in the bone marrow; released into peripheral blood | platelets (thrombocytes)
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| how long to platelets circulate? what are they removed by? | 9-12 days; liver and spleen
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| What are the 3 zones of a platelet? | peripheral, sol-gel, organelle
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| Direct contact with blood, coagulation factors interact here, stickiness | peripheral
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| microtubules and microfilaments; shape changes | sol-gel
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| platelet metabolism | organelle
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| What are the 3 main functions of a platelet? | 1. maintain vascular epithelium 2. respond to vascular injury by producing platelet plug 3. participate in plasma coagulation and help form fibrin clot
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| Reference value for normal peripheral blood platelet count | 150,000-450,000/mm^3 (ul)
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| How do you collect a satisfactory peripheral blood specimen for use in platelet counts? | venous blood; EDTA anticoagulant
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| Red blood cell diluting pipette | Capillary tube
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| Platelet diluting fluid | 1% ammonium oxalate
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| Functions of platelet diluting fluid | lyse RBCs, dilute out WBCs and platelets
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| Charge hemocytometer | pipette at a 45 degree angle, tip at edge of coverglass, squeeze a drop of fluid and capillary action will draw it in
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| How do you prevent double counting of platelets? | Don't count platelets on the bottom or right border, count them if at the top or left border
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| What is hemostasis | The process which has evolved to prevent spontaneous bleeding and stop bleeding that has been initiated by trauma
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| vasoconstriction | vascular
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| diversion of blood flow around an injured area | vascular
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| activating platelets | vascular
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| activating coagulation system when a clot is required | vascular
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| maintenance of health vascular epithelial cells by releasing an endothelial growth factor | platelet
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| stoppage of bleeding by forming platelet plug | platelet
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| creation of stable hemostatic plug | platelet
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| coagulation factors form fibrin | coagulation
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| coagulation cascade | coagulation
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| extrinsic and intrinsic pathways and common pathway | coagulation
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| plasmin degrades fibrin and reestablishes blood flow | fibrinolytic
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| promotes healing | fibrinolytic
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| PT | prothrombin time
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| used to monitor action of extrinsic system | prothrombin time
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| APTT | Activated partial thromboplastin time
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| evaluates function of intrinsic system | APTT
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| what is the anticoagulant used in a one-stage PT | sodium citrate, blue tube top
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| Whats the difference btwn serum and plasma | serum is plasma without clotting proteins
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| what type of patient sample is used in a one-stage PT | venous blood
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| typical reference value for PT | 10-13 seconds
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| What reagent is used to preform a PT; what substances in it cause it to clot? | thromboplastin - CaCl2 reagent; calcium and thromboplastin factor III
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