Molecular Biology
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| What end do nucleotides add to? | 3'
🗑
|
||||
| Adenine pairs with.... | thymine
🗑
|
||||
| Guanine pairs with... | cytosine
🗑
|
||||
| Are C-G bonds more stable or are A-T bonds more stable? | C-G because of 3 hydrogen bonds
🗑
|
||||
| A nucleotide has | nucleic acid base, sugar and a phosphate
🗑
|
||||
| The phosphodiester bond between neighboring nucleotides in DNA is | 3' to 5'
🗑
|
||||
| What are the 4 types of nitrogenous bases | adenine, guanine, thymine, cytosine
🗑
|
||||
| purines | adenine and guanine
🗑
|
||||
| pyrimidines | cytosine and thymine
🗑
|
||||
| In RNA U (uracil) replaces.... | thymine
🗑
|
||||
| More G-C bonds means | higher melting point
🗑
|
||||
| C-value and C-value paradox | genome size; genome size is not related to complexity of an organism
🗑
|
||||
| Decrease in salt concentration will....... Tm of DNA | Decrease
🗑
|
||||
| DNA can be denatured by: | low salt concentration, heat, organic solvents, High pH
🗑
|
||||
| The most abundant RNA in a cell is: | rRNA (protein translation)
🗑
|
||||
| DNA insertion in a vector shuts down the LacZ gene expression, and turns the c colony to white | Blue-white screening
🗑
|
||||
| Blue colonies | no insert
🗑
|
||||
| white colonies | contains insert
🗑
|
||||
| Chain terminating nucleotides | ddNTPs
🗑
|
||||
| What are the differences btwn sanger sequencing and PCR | ddNTPs in sanger and theres 2 primers for PCR and only 1 for DNA sequencing
🗑
|
||||
| Shows where your cloning gene is expressed | Reporter gene
🗑
|
||||
| The enzyme that cuts at a specific site is | restriction enzyme
🗑
|
||||
| DNA fingerprinting is | used to identify persons base on their unique DNA
🗑
|
||||
| Southern blot is to detect specific | DNA
🗑
|
||||
| The enzyme use in PCR is | DNA ligase
🗑
|
||||
| Which cycle starts to make two of exact length specified by the pair of primers in PCR | 3
🗑
|
||||
| cDNA is: | eukaryotic DNA with only exons that can be cloned into prokaryotes
🗑
|
||||
| Scientists use PUC18 as a gene cloning vector that includes a gene for a portion of beta-galactosidase | if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies remain white
🗑
|
||||
| To carry out sanger sequencing a mixture is needed containing | SSDNA, DNA polymerase, four deoxyribonucleotides a,t,c,g
🗑
|
||||
| What is the function of dideoxynucleotides in sanger DNA sequencing | they stop synthesis at a specific site, so the base at that site can be determined
🗑
|
||||
| a method for determining the DNA sequence of an entire genome. After a genome is cut into small fragments, each fragment is sequenced and then placed in the proper order | whole genome shotgun method
🗑
|
||||
| ddNTPs are used in which biochemical reactions | DNA sequencing
🗑
|
||||
| What is BLAST | BLAST searches for sequence matches in a nucleotide or protein database
🗑
|
||||
| You have a DNA sequence and you wish to find out whether it could encode a protein. Which BLAST program should you use? | BLASTP
🗑
|
||||
| Which of the following reporter genes cannot be used for live cell imaging? | beta-galactosidase
🗑
|
||||
| Genome-wide gene expression patterns can be studied using | microarrays
🗑
|
||||
| To use a microarray, mRNA from cells of interest are first converted to | cDNA
🗑
|
||||
| When DNA is damaged by UV light and is not repaired | neither DNA replication nor transcription can occur and the organism will probably die
🗑
|
||||
| Which of the following mechanisms is required to repair pyrimidine dimers cause by ultra violet in the presence of light in bacteria | photoreactivation
🗑
|
||||
| In methyl-directed mismatch repair, the strand that is repaired is | the non-methylated strand
🗑
|
||||
| In humans, xeroderma pigmentosum is cause by mutations in | nucleotide excision repair
🗑
|
||||
| DNA replication is | semiconservative
🗑
|
||||
| DNA polymerase I is.... | most abundant
🗑
|
||||
| DNA polymerase II is... | major enzyme for DNA replication
🗑
|
||||
| Arrange the following proteins in the proper order they participate in DNA replication | helicase, SS binding protein, primase, DNA polymerase I
🗑
|
||||
| How many replication forks would be present in the E. Coli chromosome for cells that are growing under optimal condition | two
🗑
|
||||
| DNA polymerase use their....... activity to remove a mismatched basepair | 3' to 5'
🗑
|
||||
| Proofreading by DNA polymerase involves the removal of | several bases on the newly synthesized strand of DNA
🗑
|
||||
| DNA polymerase cannot replicate | the 3' end of linear chromosomes
🗑
|
||||
| Which of the following best describes the function of telomerase at the telomere? | it adds new DNA to the longer strand of the DNA overhang
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
10209767480851378
Popular Biology sets