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Ch 10: Microbial Genetics

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Question
Answer
Bacteriocin   one of a group of bacterial proteins toxic to other bacterial cells  
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Bacteriophage   a virus that infects and replicates inside a bacterial cell  
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Biotechnology   the commercial application of genetic engineering using living organisms  
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Conjugation   the unidirectional transfer of genetic material from a live donor cell into a live recipient cell during a period of cell contact  
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Defective particle   a virus particle that does not contain the complete set of viral genes needed for viral replication  
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DNA polymerase   an enzyme that catalyzes DNA replication by combining complementary nucleotides to an existing strand  
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F factor   a plasmid containing genes for plasmid replication and conjugation pilus formation  
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Genetic engineering   the use of bacterial and microbial genetics to isolate, manipulate, recombine and express genes  
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Genetic recombination   the process of bringing together different segments of DNA  
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Genome   the complete set of genes in a virus or organism  
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Horizontal gene transfer   the movement of genes from one organism to another within the same generation  
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Induced mutation   a change in the sequence of nucleotide bases in a DNA molecule arising from a mutagenic agent used under controlled laboratory conditions  
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Mutagen   a chemical or physical agent that causes a mutation  
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Mutation   a change in the characteristic of an organism arising from a permanent alteration of a DNA sequence  
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Nucleoid   the chromosomal region of a prokaryotic cell  
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Phenotype   the physical appearance of an organism  
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Plasmid   a small, closed loop molecule of DNA apart from the chromosome that replicates independently and carries nonessential genetic information  
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Point mutation   the replacement of one base in a DNA strand with another base  
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Recombinant DNA molecule   a DNA molecule containing DNA from two different sources  
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Replication fork   the point where complementary strands of DNA separate and new complementary strands are synthesized  
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Restriction endonuclease   a type of enzyme that splits open a DNA molecule at a specific restricted point  
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Semiconservative replication   the DNA copying process where each parent (old) strand serves as a template for a new complementary strand  
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Spontaneous mutation   a mutation that arises from natural phenomena in the environment  
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Sticky end   the unpaired ends of a DNA fragment that has been cut with a restriction endonuclease  
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Transduction   the transfer of a few bacterial genes from a donor cell to a recipient cell via a bacterial virus  
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Transformation   the transfer and integration of DNA fragments from a dead and lysed donor cell to a recipient cell’s chromosome  
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Transposon   a segment of DNA that moves from one site on a DNA molecule to another site  
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Created by: drmicro
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