Biochemical Reaction
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| What are the possible outcomes of the Lysine Iron Agar test and what do they indicate about the organisms being observed? | Purple Slant/Purple butt: + for decarboxylation - K/K *anaerobic
- H2S production may be seen
Purple Slant/Yellow butt: - for decarboxylation - K/A - fermentation of glucose only
Burgundy Slant/Yellow butt: + for deamination - A/A *aerobic
🗑
|
||||
| What substances are produced by the organisms in the LIA test? | K/K - carboxyl group (-COOH) - produces cadaverine and CO2
A/A - amine group (-NH2)
K/A - no reaction
🗑
|
||||
| Why is H2S production not the most reliable indicator in biochemical testing methods? | Certain species of bacteria may have different strains that may or may not produce H2S when tested
🗑
|
||||
| What could be a potential outcome of incubating a test in a CO2 environment? | This could alter the pH of the environment, which will alter test results and lead to misidentification
🗑
|
||||
| What part of the Ornithine test do we look at for interpretation? | Color change in the butt ONLY:
- Positive = purple
- Negative = yellow
🗑
|
||||
| Which biochemical tests would be classified as deamination reactions? | - LIA
- Phenylalanine
🗑
|
||||
| What organisms can the Oxidase test help us to identify? What would be a good test to run in order to identify between them? | -Pseudomonas, Neisseria
- Cetrimide
🗑
|
||||
| When running an OF test, which container indicates the presence of acid production? | Fermentation reaction:
- acid reaction in covered and uncovered tubes (both tubes Y)
- acid reaction in covered tube only (covered tube Y, uncovered G)
Oxidation reaction:
- acid reaction in uncovered tube only (uncovered tube Y, covered tube G)
🗑
|
||||
| In the OF test, can a negative result be given? | No - the organism being observed can only be identified as a "non-utilizer", meaning that it might not utilize the specific nutrient source in that media
🗑
|
||||
| What is the main difference between the Kligler Iron Agar and the Triple Sugar Iron Agar? | The TSI Agar contains an extra nutrient source (sucrose), which helps to identify slow lactose fermenters better than the KIA
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
srstetz
Popular Science sets