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Molecular Basis of Inheritance

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Term
Definition
Chargaff-   investigated DNA composition in different species, determined DNA held genetic material  
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DNA replication-   the process of how DNA is copied  
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Structure of DNA is-   double stranded, helix  
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Sugar and phosphate groups form ___________of DNA-   backbone  
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What makes up DNA?   sugar, phosphate group, nitrogenous base  
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What two things pair to make the rungs of the ladder of DNA?   purine and pyrimidine  
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Replication/origin sites-   spots along the length of eukaryotic DNA where replication starts  
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Semi-conservative replication-   proposed by Watson and Crick, the idea that DNA has to replicate itself  
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Initiator protein-   binds to the origin sight before replication can begin  
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Helicases-   unwinds at the site of origin  
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SSB proteins-   bind to single strands to hold DNA in unwound position  
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Topoisomerase enzymes-   relieve the supercoiling upstream of DNA replication by clipping the rejoining DNA strands  
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Primase-   enzyme made of RNA that synthesizes a short sequence of nucleotides called a primer that compliments the DNA template. New DNA strand grows from this.  
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DNA polymerases-   catalyze the synthesis of DNA, takes appropriate nucleoside triphosphate and attaches it to the 3’ OH terminal of new DNA  
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What direction do enzymes add nucleotides in?   – 5’ Right Arrow 3’  
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Nucleotide-   phosphate group, sugar, nitrogenous base that, put together, make up DNA  
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Replication fork-   a y-shaped portion where parent DNA is being unwound  
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Single strand binding proteins-   bind to unpaired DNA strands to keep them from re-pairing  
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Primer-   RNA chain synthesized by primase  
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Leading strand-   The strand created by the polymerase on the top of a bubble  
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Lagging strand-   bottom strand of a bubble that has to be replicated away from the replication fork  
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DNA Ligase-   joins sugar-phosphate backbones of all Okazaki fragments  
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Okazaki fragments-   fragments of the lagging strand  
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Proof-reading and repairing DNA-   Checks the new nucleotides and replaces the incorrectly pairing nucleotides  
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Mismatch repair-   many repair enzymes remove and repair DNA mismatches after synthesis occurs  
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Nucleotide excision repair-   repairs larger-scale damage to DNA strand  
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Telomeres-   the ends of chromosomes, short nucleotide sequences repeated countless times, protect organic genes  
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Repeated sequence in humans-   TTAGGG  
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Packaging of chromatin involves-   DNA, Histones, Nucleosomes, 30 nm fiber, looped domains, metaphase chromosome  
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Histones-   protein responsible for the first level of DNA packing in chromatin  
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Nucleosomes-   histones coiled with DNA strands  
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30 nm Fiber-   interactions between the histone tails and linker DNA, pulls it all in tighter  
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Loops domains-   30 nm fiber forms loops, making a tighter fiber  
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Chromosomes-   packed histones and DNA  
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Chromatid-   one half of a chromosome  
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