Transformation of E coli with Plasmid DNA and intro to viruses phage typing
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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| method of genetic exchange involving the transfer of naked DNA from dead donor cells to living recipient cells | transformation (DNA mediated transformation)
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| ___ is restricted to bacteria that are closely related and does not occur often | transformation of genetic exchange
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| Only cells that are ___ in logarithmic stage of growth undergo transformation | competent
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| Bacteria that are ___ will readily pick up plasmid DNA | competent
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| pUC19, pBR322, pGEM and pGLO carry genes called___ that code for resistance to ampicillin | marker genes
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| ___ allow for the detection of transformed cells on media with ampicillin antibiotic | marker genes
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| the gene providing ampicillin resistance encodes for the enzyme | Beta lactamase
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| __ is an enzyme catalyzing the hydrolysis of beta lactam antibiotics (penicillins and cephalosporins) | Beta lactamase
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| the structural genes of pGLO have a gene encoding for ____ originally produced by jellyfish | green fluorescent protein
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| gells carry pGLO plasmid will produce ___ when on media containing ampicillin and arabinose | green fluorescent protein
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| __ are cells able to take in DNA from the environment from closely related cells | competent cells
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| non cellular, macromolecular comples with a nucleic acid core surrounded by a caspsid | virions
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| infective form of a viruse particle as it exists outside the host cell | virions
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| all viruses are obligate intracellular parasites or __ | hypotrophs
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| __ are ultramicroscopic life forms and infect and reproduce within specific host cells | viruses
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| biruses ata infect bacteria are called | bacteriophages
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| when bacteria and cytolytic bacteriophages are both on agar the destruction shows as windows called | plaques
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| __ means cell lysing | cytolytic
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| bacterial identification method that involves known bacteriophages to identify unknown bacteria | phage typing
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Created by:
JohnPink
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