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micro lab 22B 23A

Transformation of E coli with Plasmid DNA and intro to viruses phage typing

QuestionAnswer
method of genetic exchange involving the transfer of naked DNA from dead donor cells to living recipient cells transformation (DNA mediated transformation)
___ is restricted to bacteria that are closely related and does not occur often transformation of genetic exchange
Only cells that are ___ in logarithmic stage of growth undergo transformation competent
Bacteria that are ___ will readily pick up plasmid DNA competent
pUC19, pBR322, pGEM and pGLO carry genes called___ that code for resistance to ampicillin marker genes
___ allow for the detection of transformed cells on media with ampicillin antibiotic marker genes
the gene providing ampicillin resistance encodes for the enzyme Beta lactamase
__ is an enzyme catalyzing the hydrolysis of beta lactam antibiotics (penicillins and cephalosporins) Beta lactamase
the structural genes of pGLO have a gene encoding for ____ originally produced by jellyfish green fluorescent protein
gells carry pGLO plasmid will produce ___ when on media containing ampicillin and arabinose green fluorescent protein
__ are cells able to take in DNA from the environment from closely related cells competent cells
non cellular, macromolecular comples with a nucleic acid core surrounded by a caspsid virions
infective form of a viruse particle as it exists outside the host cell virions
all viruses are obligate intracellular parasites or __ hypotrophs
__ are ultramicroscopic life forms and infect and reproduce within specific host cells viruses
biruses ata infect bacteria are called bacteriophages
when bacteria and cytolytic bacteriophages are both on agar the destruction shows as windows called plaques
__ means cell lysing cytolytic
bacterial identification method that involves known bacteriophages to identify unknown bacteria phage typing
Created by: JohnPink
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