BI Lab 4
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| all organisms are composed of one or more cells, the cell is the basic living unit of organization, and all cells arise from pre-existing cells | cell theory
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| compound light microscope | contains two or more lenses that collect and focus light passing through a transparent specimen producing a magnified image
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| magnification | the degree to which the image of a specimen is enlarged
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| resolution | resolving power; how well specimen detail is preserved during the magnifying process
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| contrast | the ability to see specimen detail against its background
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| field of view | the circle of light one sees in the microscope when looking through the eyepiece
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| decreases, meaning a smaller area of the slide is visible | as magnification increases, the field of view _______________
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| working distance | the space between the slide and the objective
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| depth of field | vertical depth of an image that remains in sharp focus under a particular magnification
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| ocular or eyepiece | located in the upper end of the body tube. the magnification power is 10x
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| arm | functions as the handle of the microscope
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| objective lenses | attached to the revolving nosepiece. housed in one end of several steel tubes that are threaded into the revolving nosepiece
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| stage | large platform just below the revolving nosepiece
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| mechanical stage | apparatus located on the stage that holds a specimen mounted on a glass slide in place suspended over the stage opening
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| mechanical stage knobs | one moves the slide forward and back and the other moves the slide from side to side
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| coarse adjustment knob | used to move the stage up and down, so that you can bring the specimen into focus (only when using 4x)
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| fine adjustment knob | use when looking at objects using the low power objective (10x) and the high dry power objective (40x). used to bring the specimen into sharp focus
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| condenser | located between the diaphragm and the stage; converges light rays from the light source so that they pass through the specimen on the slide and into the objective lens
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| condenser adjustment knob | located underneath the stage on the left side just behind the condenser; adjusts the condenser for proper positioning to converge light rays passing through the specimen
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| iris diaphragm | lever located below the stage that regulates the amount of light passing through a specimen, thus improving the contrast of the image
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| illuminator | located below the condenser; contains the light source - a light bulb
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| base | lowermost part of the microscope and is in contact with the table
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| light switch | back of the microscope next to the electric cord mount
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| rheostat | varies the intensity of light coming from the illuminator; located on the top of the base
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| total magnification | multiply the power of the eyepiece times the power of the objective lens being used
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| permanent slide | purchased from a biological supply house and is sealed with a permanent glue
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| temporary wet mount | prepared in the lab usually using water as the suspension medium
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| parfocal | the ability of the microscope to remain in focus when switching from one objective lens to the next highest power
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| chloroplasts | function in photosynthesis and are necessary for plant life
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| cell wall | important for structural support and is not found in animal cells
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| plasma membrane | located inside the cell wall, is not visible bc it is pressed tightly against the cell wall and bc it is beyond the resolving power of the light microscope
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| cytoplasmic streaming | positions the chloroplasts toward light and distributes heat throughout the cell
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| central vacuole | provide hydrostatic pressure or turgor within individual cells
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| turgor | hydrostatic pressure
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| nucleus | control center of the cell; houses the DNA
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| plasma membrane | surrounds the cell and regulates what moves into and out of the cell
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| cytoplasm | the jelly-like material found between the cell's nucleus and plasma membrane
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| organelles | structures which perform specific cell functions
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| stereomicroscope | has two eyepieces that give a three-dimensional view of the specimen
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Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
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