Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Compound Light Microscopes | 1. Brightfield (used in high school)
2. Phase Contrast
3. Nomarski
4. Flourescence
5. Confocal
6. Dissecting microscope
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| Phase Contrast | Alignment of light beam that lets us see organelles >> highly refractive structure
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| Brightfield | 1. 2 lenses: Eyepiece (10x)
2. 3 Objectives: Scanning (4x), Low (10x), High (40x)
**Image appearance: Inversion, reversal
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| Resolving Power | The higher the resolving power, the more detail (but less objects in field of view)
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| Field of View | Diameter of view in which specimens are observed.
Low: 1.5 mm or 1500 micrometers
High: 0.375mm or 375 micrometers
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| TM | Total magnification
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| Normarski | Differential interference suited for viewing LIVE specimens
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| Fluorescence | Certain cellular structures tagged (dyed) w/fluorescent molecules
**Colored!
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| Confocal | Lasers as light source
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| Dissecting Microscope | 1. External structures of plants and animals
2. Aka stereomicroscope
3. 3D image
4. No reversal or inversion
5. Bifocal
6. 1 objective (50x)
7. Range of TM: 6-50x
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| Electron Microscope | 1. View detailed structures of cell-liked organelles
2. Magnification 250,000x
3. Beam of electrons instead of light
4. Non-living!
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| Scanning Electron Microscope (SEM) | Beam of electrons back and forth across the surface
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| Scanning Tunneling Microscope | 1. Views DNA, which repairs enzympes
2. Atoms of NaCl crystal
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| Transmission Electron Microscope | 1. Cross-section
2. Beam of electrons thru THIN specimen (0.1 micro)
3. 3D
4. Black and white
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Created by:
jubie
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