quiz 1- microscopy
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Magnification | How much larger a specimen’s image appears compared to its actual size.
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| Contrast | How well the specimen stands out from the bright background
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| Resolution/resolving power | the ability to see details and distinguish structures.
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| What do Most light microscopes have a RP of ? | 0.2 µm
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| Reflection | light bounces back off the object.
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| Absorption | light waves are taken up by the object.
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| Transmission | light passes through the object.
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| Refraction | as light passes from one medium to another it changes speed and this makes the light bend.
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| Compound light microscope | Uses a series of lenses to collect and focus light as it is transmitted through the specimen on a slide.
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| Stereo dissecting light microscope | used to observe specimens in 3-D
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| Electron microscope | - use a beam of electrons to create the image.
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| Transmission electron microscope (TEM) | used to visualize internal cellular structures 10,000-100,000X.
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| Scanning electron microscope (SEM)- | used to visualize external structures in 3-D. Can magnify image 10,000-50,000X
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| NUMERICAL APERTURE | Represents the angle of light refracted
•Measures the amount of light gathered by the objective lens
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| RESOLVING POWER | represents the size of the smallest object that can be seen with that objective
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Created by:
Virajasaur
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