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quiz 1- microscopy

Magnification How much larger a specimen’s image appears compared to its actual size.
Contrast How well the specimen stands out from the bright background
Resolution/resolving power the ability to see details and distinguish structures.
What do Most light microscopes have a RP of ? 0.2 µm
Reflection light bounces back off the object.
Absorption light waves are taken up by the object.
Transmission light passes through the object.
Refraction as light passes from one medium to another it changes speed and this makes the light bend.
Compound light microscope Uses a series of lenses to collect and focus light as it is transmitted through the specimen on a slide.
Stereo dissecting light microscope used to observe specimens in 3-D
Electron microscope - use a beam of electrons to create the image.
Transmission electron microscope (TEM) used to visualize internal cellular structures 10,000-100,000X.
Scanning electron microscope (SEM)- used to visualize external structures in 3-D. Can magnify image 10,000-50,000X
NUMERICAL APERTURE Represents the angle of light refracted •Measures the amount of light gathered by the objective lens
RESOLVING POWER represents the size of the smallest object that can be seen with that objective
Created by: Virajasaur