prokaryotic DNA replication
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| What is the point where the two parental DNA strands separate to allow replication? | replication fork
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| What is the multi-protein machine that mediateds bacterial DNA replication? | replisome
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| What is the unique site on chromosomal DNA where replcation begins? | origin of replication (ORI)
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| What is the ORI of E. coli ? | oriC
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| What is the enzyme that unwinds a DNA or RNA double helix? | helicase
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| What is the fragment of E. coli DNA polymerase I that lacks the 5' to 3' exonuclease activity of the parent enzyme? | Klenow fragment
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| What is the strand that is made discontinuously in DNA replication? | lagging strand
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| What is the strand that is made of continuously in DNA replication? | leading strand
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| What is the enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond? | ligase
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| What is the enzyme that catalyzes the formation of an RNA primer during DNA replication? | primase
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| What is the enzyme that plays a role in primer removal and gap filling between Okazaki fragments in E. coli DNA replicaton? | DNA polymerase I
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| What is the enzme that catalyzes DNA strand elongation in E.coli replication? | DNA polymerase III
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| What is the protein that binds to single-stranded DNA and keeps it from base pairing with complemetary strand during DNA replication? | SSB
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| What is the protein that helps tether DNA polymerase to the DNA during replication to allow highly processive synthesis? | sliding clamp
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| What are the small DNA fragments created by discontinuous synthesis of the lagging strand? | Okazaki fragments
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| What is the enzyme that introduces negative supercoil into DNA during E. coli DNA replication? | gyrase
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