UALR Microbiology- Dr. Ali
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| What does it mean to hydrolyze? | To break bonds with water
🗑
|
||||
| Why must bacteria hydrolyze larger molecules? | To allow nutrients in the cell
🗑
|
||||
| Hydrolysis is accomplished by the secretion of what? | exoenzymes or hydrolases
🗑
|
||||
| 4 extracellular enzymes: | Starch; Lipid; Casein; Gelatin
🗑
|
||||
| Hydrolysis test is performed using what 2 components? | Starch agar plate and iodine
🗑
|
||||
| Gelatin is a __________ protein. | incomplete
🗑
|
||||
| At what temperature is gelatin solid? | Less than 25 degrees Celsius
🗑
|
||||
| At what temperature is gelatin liquid? | More than 25 degrees Celsius
🗑
|
||||
| How do you know if a Catalase test is positive? | The formation of gas/ bubbles
🗑
|
||||
| Where can Oxidase be found? | In the Mitochondria
🗑
|
||||
| How do you know if your bacteria is Oxidase positive? | Color change to Deep Purple within 20 seconds of being streaked on test slide
🗑
|
||||
| How do you test for Catalase activity? | Add H2O2 (Hydrogen Peroxide) to bacteria.
🗑
|
||||
| What does "SIM" stand for? | S- Sulfide
I- Indole
M- Motility
🗑
|
||||
| What does the SIM test media contain? | Amino acids (tryptophan); Peptones (source of cysteine); Ferrous Ammonium
🗑
|
||||
| How do you know if your SIM results are Sulfide positive? | The appearance of black precipitate (indicates the presence of H2S)
🗑
|
||||
| How do you know if your SIM results are indole positive? | Kovac's reagent turns red on top of agar
🗑
|
||||
| What does MR-VP stand for? | Methyl Red and Voge's Proskauer
🗑
|
||||
| What does MR-VP test for? | Fermentation end products between species
🗑
|
||||
| What color and what pH indicate positive MR test? | Red color and pH of 5 or less (pH4); indicates mixed acid
🗑
|
||||
| The VP test detects the presence of ____________. | acetoin
🗑
|
||||
| What color indicates a positive VP test? | Rose
🗑
|
||||
| Litmus Milk is made up of what 3 different metabolized components? | Latose; Casein; Litmus
🗑
|
||||
| Litmus turns what color when acidic? | Red
🗑
|
||||
| Litmus turns what color when neutral? | Purple
🗑
|
||||
| Litmus turns what color when alkaline? | Blue
🗑
|
||||
| If fissures appear in the solidified (curd) of the Litmus Milk, what has been produced? | Gas
🗑
|
||||
| Litmus Milk that has turned pink, indicates what kind of reaction? | Lactose Fermentation (acidic)
🗑
|
||||
| Litmus Milk that has turned a brownish color with a purple band on top, has experienced what kind of reaction? | Proteolysis
🗑
|
||||
| Litmus Milk that has turned white with a purple band on top, indicates what type of reaction? | Litmus Reduction
🗑
|
||||
| The citrate test determines what? | Whether or not the organism can use citrate as its sole carbon source.
🗑
|
||||
| A positive citrate test will turn from what color to what color? | Green to Blue
🗑
|
||||
| What is the pH indicator called for the citrate test? | Bromthymol Blue
🗑
|
||||
| During the Nitrate reduction test, the appearance of what color indicates the presence of No2-? | Red
🗑
|
||||
| The Nitrate Reduction test, tests for what? | To see if bacterium can use nitrate as a final electron acceptor during anaerobic respiration
🗑
|
||||
| If positive for Nitrate utilization, then there are three possible positive end products. What are they? | N2 (gas); NO2- (nitrite);NH3 (ammonia)
🗑
|
||||
| Antisepsis | Reduction in the number of microorganisms and viruses; particularly potential pathogens, on living tissue
🗑
|
||||
| Aseptic | Refers to an environment or procedure free of pathogenic microorganisms.
🗑
|
||||
| What do the suffixes "-cide" and "-cidal" indicate? | the destruction of a type of microbe
🗑
|
||||
| De-germing | The removal of microbes by mechanical means
🗑
|
||||
| Disinfection | Destruction of most microorganisms and viruses on non-living tissue
🗑
|
||||
| Pasteurization | The use of heat to destroy pathogens and to reduce the number of spoilage in foods and beverages.
🗑
|
||||
| 6 Efficiency Factors of Chemical Agents; | Concentration; Length of exposure; Environmental Conditions; Material type; Age of culture
🗑
|
||||
| Antibiotics originate from; | Living organisms
🗑
|
||||
| Synthetics originate from: | Laboratories
🗑
|
||||
| 6 physical methods of microbial control: | Heat; autoclave (pasteurization); Filtration; Cold; Desiccation and high osmotic pressure; Radiation
🗑
|
||||
| Hyertonic | High solute; Low solvent (cell loses water)
🗑
|
||||
| Plasmolysis | when cell loses water
🗑
|
||||
| Hypotonic | Low solute; High solvent (cell absorbs water)
🗑
|
||||
| Plasmoptysis | Cell absorbs water and swells
🗑
|
||||
| Isotonic | No movement; equal solute and solvent; equilibrium
🗑
|
||||
| 2 forms of mutagenic electromagnetic radiation: | ionizing and non-ionizing radiation
🗑
|
||||
| Why test water? | Water plays a huge role in the transmission of disease
🗑
|
||||
| 4 classes of communicable diseases: | food and water; airborne; arthropods; contact
🗑
|
||||
| Genotype vs. Phenotype | Genotype= Genetic/ DNA
Phenotype= Observable characteristics
🗑
|
||||
| Type "O" blood is the universal _____________ | donor
🗑
|
||||
| Type "AB" blood is the universal _____________ | Acceptor
🗑
|
||||
| Reaction to anti-B indicates what blood type? | B
🗑
|
||||
| Reaction to Anti-A indicates what blood type? | A
🗑
|
||||
| No reaction to anti-A or anti-B indicates what blood type? | O
🗑
|
||||
| if a person if Rh+ they have what antigen? | Antigen D
🗑
|
||||
| Type 1 Hypersensitivity | Antibody to antigen reaction
🗑
|
||||
| Type 2 Hypersensitivity | Antibody to antigen on RBC
🗑
|
||||
| UV light is an example of what type of radiation? | Non-ionizing
🗑
|
||||
| X-rays and gamma rays are examples of what type of radiation? | Ionizing
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
BabyNurse17
Popular Biology sets