BZ 310- Lab Final
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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three levels of cell cultures | culture, subculture, cell line
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cell counting | dilution x 1000 x (cells counted)/(mL)
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.025%= | .25mg/mL
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freezing medium contains what and why? | DMSO to prevent freezing damage
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growth medium | Grace's medium, 10% bovine serum
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three growth phases | lag, log, plateau
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three things used to prevent contamination | laminar flow cabinets, 70% ethanol, UV lights
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most common problem for cell culture | mycoplasma from the mouth (breath)
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flow cabinets cannot be used for | pathogens since they flow outward
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Lowry | one part detects tryptophan and tyrosine, other part detects peptide bonds
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Bradford | coomassie brilliant blue color change proportional to the concentration of protein
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Folin-Ciocalteau | (Lowry) detects trp/tyr by phosphomolybdate salts by reacting with them
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Biuret reaction | (Lowry) turns purple when copper sulfate reacts with peptide bonds
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Created by:
melaniebeale
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