BZ 310- Lab Quiz 3
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| what was plasmid 1? | pBS
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| what was plasmid 2? | pUC7K
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| two types of gel for electrophoresis | poly-acrylamide and agarose
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| what makes DNA visible on gels? | ethidium bromide
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| how to make cells competent? (2) | heat or CaCl2
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| what did plasmid 1 grow on and not grow on? | grew on ampicillin, not kanamycin (pBS)
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| what did plasmid 2 grow on? | both kanamycin and ampicillin (pUC7K)
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| CFU/mL | # colonies per mL plated / dilution (1/10 etc)
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| how to find g/plasmid | 700 g/mol (1 base pair) x # base pairs / 6.022E23
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| fraction transformed | CFU/mL transformed / CFU/mL control
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| transformation efficiency | CFU/mL / ug plasmid added
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| what makes glycerinated myofibers contract? | ATP, Mg, Ca, K
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| what is added to observe skeletal muscle and why? | glycerin, removed membrane but leaves contractile apparatus intact
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| what did we add to sperm and why? | Triton X-100 to removes membranes, DTT stabilize ATP binding site
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| overall photosynthesis reaction | 6CO2 + 12H2O -> C6H12O6 + 6O2 + 6H2O
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| where do the oxygens in O2 product of photosynthesis come from? | the H2O
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| what color was DCIP when reduced? | clear
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| what color was DCIP when oxidized? | blue
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| DCIP | in place of NADPH for hill reaction
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| order of hill reaction | PS II, PQ, PS I, ferredoxin, NADPH
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| where are cells coming from this week? | SF9 insect cells (fall armyworm)
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| what will be used this week to prevent freezing damage? | DMSO
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| how will we make a sterile environment this week? | UV
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| what will we use this week to prevent contamination? | flow cabinets
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| each muscle cell is a | fiber
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| the striated appearance of muscle fibers is caused by | actin and myosin overlap
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| photosynthesis requires light, H2O, CO2, and | chloroplasts
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| why use insect cells? | less sensitive to temperature and CO2 concentration
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