BZ 310- Lab Quiz 3
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| partition coefficient | lower= will pass through the membrane (lipid soluble)
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| chloroform-methanol | lipid solvent
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| Triton X-100 | nonionic detergent
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| Sodium dodecyl sulfate | ionic detergent
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| most water soluble compound in list | n-propanol
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| least water soluble compound in list | glycerol
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| 5 properties that make E. coli DH5-alpha safe | pro/thi auxotroph, no polysaccharide layer, cell wall defects, lack of enzyme that degrades plasmid DNA, lack of ability to recombine DNA
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| EDTA | destabilizes cell wall and protects DNA from degradation
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| restriction enzymes depend on (2) | Mg2+ ions and neutral pH
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| find ug DNA | absorbance at 260 (DNA) x 50 ug/mL x 15 dilution x 1mL/1000 uL x 50 uL
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| absorbance is directly proportional to | concentration of the sample
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| hypotonic | less solutes outside of cell, cell bursts
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| bacterial trait helpful to molecular biologist but not doctor | altered so it cannot make one sick
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| the two different types of DNA present in bacteria are similar in what sense? | double stranded, encode for genes
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| wavelength DNA | 260nm
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| wavelength protein | 280nm
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| how did we differentiate between transformed and non-transformed cells? | whether or not they grew on antibiotics
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| two types of competent cells | yeast (naturally), any cell treated w/ CaCl2
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| crenate | hypertonic
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| calculate CFU/mL | colonies/mL/(1/dilution)
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Created by:
melaniebeale
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