MMBio Ch.7 Enzymes
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Type II restriction endonuclease | cleaves DNA at specific base sequences
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| DNA ligase | joins two DNA molecules or fragments
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| DNA polymerase I | Fills single-stranded gapes in duplex DNA by stepwise addition of nucleotides to 3' ends
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| Reverse transcriptase | Makes a DNA copy of an RNA molecule
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| Polynucleotide kinase | Adds a phosphate to the 5'-OH end of a polynucleotide, to label it or permit ligation
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| Terminal transferase | Adds homopolymer tails to the 3'-OH ends of a linear duplex
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| Exonuclease III | Removes nucleotide residues from the 3' ends of DNA strand
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| Bacteriophage (lambda) exonuclease | removes nucleotides from the 5' ends of a duples to expose single-stranded 3' ends
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| Alkaline phosphatase | removes terminal phosphates from the 5' end, the 3' end, or both
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