click below
click below
Normal Size Small Size show me how
MMBio Ch.7 Enzymes
Term | Definition |
---|---|
Type II restriction endonuclease | cleaves DNA at specific base sequences |
DNA ligase | joins two DNA molecules or fragments |
DNA polymerase I | Fills single-stranded gapes in duplex DNA by stepwise addition of nucleotides to 3' ends |
Reverse transcriptase | Makes a DNA copy of an RNA molecule |
Polynucleotide kinase | Adds a phosphate to the 5'-OH end of a polynucleotide, to label it or permit ligation |
Terminal transferase | Adds homopolymer tails to the 3'-OH ends of a linear duplex |
Exonuclease III | Removes nucleotide residues from the 3' ends of DNA strand |
Bacteriophage (lambda) exonuclease | removes nucleotides from the 5' ends of a duples to expose single-stranded 3' ends |
Alkaline phosphatase | removes terminal phosphates from the 5' end, the 3' end, or both |