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MMBio Ch.7 Enzymes

TermDefinition
Type II restriction endonuclease cleaves DNA at specific base sequences
DNA ligase joins two DNA molecules or fragments
DNA polymerase I Fills single-stranded gapes in duplex DNA by stepwise addition of nucleotides to 3' ends
Reverse transcriptase Makes a DNA copy of an RNA molecule
Polynucleotide kinase Adds a phosphate to the 5'-OH end of a polynucleotide, to label it or permit ligation
Terminal transferase Adds homopolymer tails to the 3'-OH ends of a linear duplex
Exonuclease III Removes nucleotide residues from the 3' ends of DNA strand
Bacteriophage (lambda) exonuclease removes nucleotides from the 5' ends of a duples to expose single-stranded 3' ends
Alkaline phosphatase removes terminal phosphates from the 5' end, the 3' end, or both
Created by: ksheehy96