Microorganisms
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Giesma for H. Pylori | stains for helicobacter pylori
diff quick solution I
diff quick solution II
Acetic water, then check with microscope
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| Giesma for H. Pylori | H. pylori.................DARK BLUE
Other bacteria.......BLUE
Nuclei....................DARK BLUE
Cytoplasm...............PINK
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| Brown-Hopps Modified Gram Stain | stains gram positive and negative bacteria
crystal violet (gram positive)
gram iodine(mordant)
blot paper, acetone (decolorize neg)
basic fuchsin->gallego->acetone->picric acid acetone
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| Brown-Hopps Modified Gram Stain | Gram (+).........BLUE
Gram(-)...........RED
Background.....YELLOW
Nuclei..............LIGHT RED
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| Auramine-Rhodamine Fleorescene | stains for Mycobacterium Tuberculosis or other AFB
Auromine-Rhodamnie B solution-> acid alcohol-> Eriochrome Black T
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| Auramine-Rhodamine Fleorescene | AFB..................reddish yellow fluorescene
Background......BLACK
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| Fite Acid Fast Stain for Leprosy | demonstrates mycobacterium leprae
xylene with peanut oil-> ZN carbol fuchsin-> acid alcohol-> methyl blue
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| Fite Acid Fast Stain for Leprosy | M leprae...............RED
Background...........BLUE
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| Acid Fast Kinyoun Acid Fast Stain | Kinyoun Carbol-Fuchsin -> Acid Alcohol (to differentiate)-> Methylene Blue (counterstain)
AFB....................BRIGHT RED
Background.........BLUE
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| Ziehl-Neelsen for AFB | Ziehl Neelson Carbol Fuchsin (uses ethyl alcohol)
acid alcohol-> methylene blue
AFB....................BRIGHT RED
Background.........BLUE
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| Hotchkiss-Mcmanus PAS for Fungus | demonstrates fungus
Fungi.................ROSE
Background.......GREEN
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| FUNGUS STAINS | PASF
CHROMIC ACID-SCHIFF STAIN
GRIDLEY
GROCOTT METHANAMINE SILVER NITRATE
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| Grocott Methanamine- Silver Nitrate Fungus Stain | chromic acid is the oxidizer for the fungal cell wall
stains for pneumocystis jirovecii
chromic acid->sodium bisulfate-> pre-heated methanamine silver-> gold chloride-> sodium thiosulfate -> light green
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| Grocott Methanamine- Silver Nitrate Fungus Stain | Fungi......................BLACK
Mucin......................TAUPE
Background..............GREEN
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| SPIROCHETE STAINS | WARTHIN-STARRY
DIETERLE
STEINER & STEINER
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| Warthin-Starry for Spirochetes | 2% Silver nitrate (developer)
1% Silver nitrate (impregnation)
Developer (gelatin, hydroquinine, 2% silver nitrate)
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| Warthin-Starry for Spirochetes | Spirochetes & bacteria.........BLACK
Background..........................YELLOW/BROWN
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| Steiner | demonstrates spirochetes, helicobacter, and legionella
uses gum mastic
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| Steiner | Spirochetes, H. pylori, L. Pneumophila,
other nonfilamentous bacteria...............DARK BROWN TO BLACK
Background............................................LIGHT YELLOW
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You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
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Created by:
burkan2
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