connective tissue stains
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Osmium Tetroxide | only chemical method for staining fat
fat becomes insoluble in alcohols and xylene
tissue can be processed for paraffin embedding
can counterstain with H&E or masson trichrome
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| Toluidine Blue | Stains Mast cells
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| Mast cells | Play a key role in inflammation and allergic reactions
associated with autoimmune disorders
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| Sudan Black B | most sensitive of lipid dyes
use propylene glycol
counterstained with nuclear fast red
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| Oil red O | method for neutral fats
used on frozen sections fixed with formaldehyde
Harris hematoxylin blued in ammonia water
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| Periodic -Acid Methenamine Silver | For Basement Membranes
Used to detect disease in glomerular basement membrane
uses periodic acid, methanimine solution, gold chloride, sodium thiosulfate, and light green counterstain
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| Periodic -Acid Methenamine Silver | basement membrane ........black
background.........................green
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| Osmium Tetroxide | Fat....................................black
other tissue elements.......depends on counter method
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| Toluidine Blue | mast cells....... rose
background..... blue
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| Sudan Black B | Fat................blue/black
Nuclei............red
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| Oil red O | Lipids........ red
Nuclei.........blue
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| Methenamine silver | silver nitrate and methenamine
Working solution contains methenamine silver, distilled water and borax
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| Mallory PTAH Technique | demonstrates cross striations and fibrin
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| Mallory PTAH Technique | cross striations, fibrin........blue
nuclei................................blue
collagen............................red/brown
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| Gordon and Sweets | Stains for reticular fibers
oxidizes in 1% potassium permanganate
****bleaches in oxalic acid****
sensitizes in ferric ammonium sulfate
uses ammoniacal silver
formalin
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| Gordon and Sweets | reticulin........black
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| Ammoniacal Silver Solution | 5ml Silver nitrate
ammonium hydroxide until no precipitate
5ml sodium hydroxide
ammonium hydroxide until faint cloudiness
dilute remaining solution to 50ml with distilled water
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| Gomori for reticular fibers | oxidize in potassium permanganate
differentiate in **potassium metabisulfate**
sensitize in ferric ammonium sulfate
ammoniacal silver solution reduced in formalin
gold chloride> potassium metabisulfate>sodium thiosulfate
optional nuclear fast red co
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| Gomori for reticular fibers | reticulin...............black
collagen..............taupe
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| Movat Pentachrome | nuclei and elastic fibers...............black
collagen...........................................yellow
ground substance, mucin..............................blue
fibrinoid, fibrin.........................intense red
muscle...............red
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| Verhoeff Van Gieson | for elastic fibers
ferric chloride is used in the primary stain as well as a differentiater
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| Verhoeff stain | alcoholic hematoxylin
ferric chloride
lugol iodine
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| Van Gieson stain | acid fuchin
picric acid
*stains collagen RED
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| Vehoeff Van Gieson | elastic fibers.... blue/black
nuclei...............blue/black
collagen...........red
other tissue......yellow
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| Gomori Trichrome | chromotrope 2r
fast green or aniline blue
phosphotungstic acid
glacial acetic acid
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| Gomori Trichrome | nuclei.........................black
cytoplasm, keratin, muscle fibers........red
collagen,mucin..........green or blue
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| Masson Trichrome | Bouins,
Weigherts,
Biebrich scarlet-acid fuchsin
phosphomolybdic/phosphotungstic acid
aniline blue (use fast green when cologne is predominant)
acetic acid
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| Masson Trichrome | nuclei......................... black
keratin, muscle, cytoplasm....red
collagen, mucin............blue
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| Gomori Aldehyde Fuchsin | Elsatic Stain
elastic fibers..........blue/purple
other tissue....................green
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| Aldehyde fuchsin | hydrochloric acid and paraldehyde added to alcoholic solution of basic fuchsin
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