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special stains
connective tissue stains
| Term | Definition |
|---|---|
| Osmium Tetroxide | only chemical method for staining fat fat becomes insoluble in alcohols and xylene tissue can be processed for paraffin embedding can counterstain with H&E or masson trichrome |
| Toluidine Blue | Stains Mast cells |
| Mast cells | Play a key role in inflammation and allergic reactions associated with autoimmune disorders |
| Sudan Black B | most sensitive of lipid dyes use propylene glycol counterstained with nuclear fast red |
| Oil red O | method for neutral fats used on frozen sections fixed with formaldehyde Harris hematoxylin blued in ammonia water |
| Periodic -Acid Methenamine Silver | For Basement Membranes Used to detect disease in glomerular basement membrane uses periodic acid, methanimine solution, gold chloride, sodium thiosulfate, and light green counterstain |
| Periodic -Acid Methenamine Silver | basement membrane ........black background.........................green |
| Osmium Tetroxide | Fat....................................black other tissue elements.......depends on counter method |
| Toluidine Blue | mast cells....... rose background..... blue |
| Sudan Black B | Fat................blue/black Nuclei............red |
| Oil red O | Lipids........ red Nuclei.........blue |
| Methenamine silver | silver nitrate and methenamine Working solution contains methenamine silver, distilled water and borax |
| Mallory PTAH Technique | demonstrates cross striations and fibrin |
| Mallory PTAH Technique | cross striations, fibrin........blue nuclei................................blue collagen............................red/brown |
| Gordon and Sweets | Stains for reticular fibers oxidizes in 1% potassium permanganate ****bleaches in oxalic acid**** sensitizes in ferric ammonium sulfate uses ammoniacal silver formalin |
| Gordon and Sweets | reticulin........black |
| Ammoniacal Silver Solution | 5ml Silver nitrate ammonium hydroxide until no precipitate 5ml sodium hydroxide ammonium hydroxide until faint cloudiness dilute remaining solution to 50ml with distilled water |
| Gomori for reticular fibers | oxidize in potassium permanganate differentiate in **potassium metabisulfate** sensitize in ferric ammonium sulfate ammoniacal silver solution reduced in formalin gold chloride> potassium metabisulfate>sodium thiosulfate optional nuclear fast red co |
| Gomori for reticular fibers | reticulin...............black collagen..............taupe |
| Movat Pentachrome | nuclei and elastic fibers...............black collagen...........................................yellow ground substance, mucin..............................blue fibrinoid, fibrin.........................intense red muscle...............red |
| Verhoeff Van Gieson | for elastic fibers ferric chloride is used in the primary stain as well as a differentiater |
| Verhoeff stain | alcoholic hematoxylin ferric chloride lugol iodine |
| Van Gieson stain | acid fuchin picric acid *stains collagen RED |
| Vehoeff Van Gieson | elastic fibers.... blue/black nuclei...............blue/black collagen...........red other tissue......yellow |
| Gomori Trichrome | chromotrope 2r fast green or aniline blue phosphotungstic acid glacial acetic acid |
| Gomori Trichrome | nuclei.........................black cytoplasm, keratin, muscle fibers........red collagen,mucin..........green or blue |
| Masson Trichrome | Bouins, Weigherts, Biebrich scarlet-acid fuchsin phosphomolybdic/phosphotungstic acid aniline blue (use fast green when cologne is predominant) acetic acid |
| Masson Trichrome | nuclei......................... black keratin, muscle, cytoplasm....red collagen, mucin............blue |
| Gomori Aldehyde Fuchsin | Elsatic Stain elastic fibers..........blue/purple other tissue....................green |
| Aldehyde fuchsin | hydrochloric acid and paraldehyde added to alcoholic solution of basic fuchsin |
Created by:
burkan2
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