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special stains

connective tissue stains

TermDefinition
Osmium Tetroxide only chemical method for staining fat fat becomes insoluble in alcohols and xylene tissue can be processed for paraffin embedding can counterstain with H&E or masson trichrome
Toluidine Blue Stains Mast cells
Mast cells Play a key role in inflammation and allergic reactions associated with autoimmune disorders
Sudan Black B most sensitive of lipid dyes use propylene glycol counterstained with nuclear fast red
Oil red O method for neutral fats used on frozen sections fixed with formaldehyde Harris hematoxylin blued in ammonia water
Periodic -Acid Methenamine Silver For Basement Membranes Used to detect disease in glomerular basement membrane uses periodic acid, methanimine solution, gold chloride, sodium thiosulfate, and light green counterstain
Periodic -Acid Methenamine Silver basement membrane ........black background.........................green
Osmium Tetroxide Fat....................................black other tissue elements.......depends on counter method
Toluidine Blue mast cells....... rose background..... blue
Sudan Black B Fat................blue/black Nuclei............red
Oil red O Lipids........ red Nuclei.........blue
Methenamine silver silver nitrate and methenamine Working solution contains methenamine silver, distilled water and borax
Mallory PTAH Technique demonstrates cross striations and fibrin
Mallory PTAH Technique cross striations, fibrin........blue nuclei................................blue collagen............................red/brown
Gordon and Sweets Stains for reticular fibers oxidizes in 1% potassium permanganate ****bleaches in oxalic acid**** sensitizes in ferric ammonium sulfate uses ammoniacal silver formalin
Gordon and Sweets reticulin........black
Ammoniacal Silver Solution 5ml Silver nitrate ammonium hydroxide until no precipitate 5ml sodium hydroxide ammonium hydroxide until faint cloudiness dilute remaining solution to 50ml with distilled water
Gomori for reticular fibers oxidize in potassium permanganate differentiate in **potassium metabisulfate** sensitize in ferric ammonium sulfate ammoniacal silver solution reduced in formalin gold chloride> potassium metabisulfate>sodium thiosulfate optional nuclear fast red co
Gomori for reticular fibers reticulin...............black collagen..............taupe
Movat Pentachrome nuclei and elastic fibers...............black collagen...........................................yellow ground substance, mucin..............................blue fibrinoid, fibrin.........................intense red muscle...............red
Verhoeff Van Gieson for elastic fibers ferric chloride is used in the primary stain as well as a differentiater
Verhoeff stain alcoholic hematoxylin ferric chloride lugol iodine
Van Gieson stain acid fuchin picric acid *stains collagen RED
Vehoeff Van Gieson elastic fibers.... blue/black nuclei...............blue/black collagen...........red other tissue......yellow
Gomori Trichrome chromotrope 2r fast green or aniline blue phosphotungstic acid glacial acetic acid
Gomori Trichrome nuclei.........................black cytoplasm, keratin, muscle fibers........red collagen,mucin..........green or blue
Masson Trichrome Bouins, Weigherts, Biebrich scarlet-acid fuchsin phosphomolybdic/phosphotungstic acid aniline blue (use fast green when cologne is predominant) acetic acid
Masson Trichrome nuclei......................... black keratin, muscle, cytoplasm....red collagen, mucin............blue
Gomori Aldehyde Fuchsin Elsatic Stain elastic fibers..........blue/purple other tissue....................green
Aldehyde fuchsin hydrochloric acid and paraldehyde added to alcoholic solution of basic fuchsin
Created by: burkan2