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effect of pH , temperature and heat denaturation on Catalase activity

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Question
Answer
The substrate used in these activities is...   Hydrogen Peroxide  
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The enzyme used in this investigation is..   Catalase  
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The source of the enzyme is ..   Radish or Celery or Liver  
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To keep pH constant...   Use a buffer solution  
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To keep temperature constant ...   Use a water bath  
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To vary the temperature use..   Water baths at different temperatures  
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The volume of foam produced is...   a measure of the rate of enzyme activity  
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What gas is released to cause the foam to form?   Oxygen  
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In relation to enzyme explain the term optimum.   the condition at which it works best  
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If the pH is too high or too low..   the enzyme may be denatured.  
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A denatured enzyme has..   lost its shape and cannot function  
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Boiling an enzyme causes it to become..   denatured  
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Created by: PDST Biology
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