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Enzymes
effect of pH , temperature and heat denaturation on Catalase activity
| Question | Answer |
|---|---|
| The substrate used in these activities is... | Hydrogen Peroxide |
| The enzyme used in this investigation is.. | Catalase |
| The source of the enzyme is .. | Radish or Celery or Liver |
| To keep pH constant... | Use a buffer solution |
| To keep temperature constant ... | Use a water bath |
| To vary the temperature use.. | Water baths at different temperatures |
| The volume of foam produced is... | a measure of the rate of enzyme activity |
| What gas is released to cause the foam to form? | Oxygen |
| In relation to enzyme explain the term optimum. | the condition at which it works best |
| If the pH is too high or too low.. | the enzyme may be denatured. |
| A denatured enzyme has.. | lost its shape and cannot function |
| Boiling an enzyme causes it to become.. | denatured |