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Microbiology Chapter 9: Biotechnology and DNA Technology

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Term
Definition
restriction enzymes   DNA-cutting enzymes that can form blunt or sticky ends.  
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vector   A general term for a self-replicating DNA molecule that can be used as a carrier to move a gene from one organism to another.  
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DNA ligase   An enzyme that links short pieces of DNA into longer pieces. It is used when creating recombinant DNA molecules and also when joining Okazaki fragments during DNA replication.  
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DNA polymerase   An enzyme that links nucleotides together to form a complementary strand of DNA during DNA replication.  
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plasmid   The most common cloning vector used in genetic engineering.  
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recombinant DNA   DNA that is made from an mRNA template.  
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