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MicroBiology

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Microbiology   study of microorganisms   study of microorganisms  
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Microorganisms (microbes)   organisms that are too small to be seen with naked eye   organisms that are too small to be seen with naked eye  
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Many microbes are helpful   -basis for food chains in aquatic environments -breakdown waste in soil -play roles in photosynthesis -aid in synthesis of vitamins in humans -Few are pathogenic (disease-causing)   -produce: ethanol, acetone, vinegar, cheese, bread, cellulose & insulin  
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Disease   condition of the body or of one of its parts that impairs normal functioning   is a particular abnormal, pathological condition that affects part or all of an organism. It is often construed as a medical condition associated with specific symptoms and signs  
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Microbes are divided into 6 subgroups   bacteria, archaea, fungi, protozoa, viruses & algae   bacteria, archaea, fungi, protozoa, viruses & algae  
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Prokaryotes   lack a nucleus/ internal membrane bound structures   bacteria & archaea are prokaryotes  
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Eukaryotes   have nucleus/ organelles are membrane bound   fungi, protozoa & algae are Eukaryotes  
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Viruses are   acellular (no cell) -extremely smaller than bacteria   they are only nucleic acid (DNA or RNA) wrapped in protein  
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Bacteria   Have several shapes like rods, spherical, helical, stars, commas or square   -can move -can live in temperatures of 20°C & some can live in temperatures of 110°C -can survive in extreme acid or alkalinity  
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Archaea   Prokaryote that resemble bacteria superficially   they can live in extreme environments  
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Methanogens   make methane as waste product   a methane forming archaea  
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Extreme halophiles   live in extremely salty environments (Great Salt Lake & Dead Sea)    
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Extreme thermophiles   live in hot sulfurous waters (Yellowstone National Park) Not known to cause disease in humans    
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Algae   are Eukaryotes -unicellular & microscopic or many cells macroscopic -   Live in in fresh & salt water environments  
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Fungi   -Eukaryote   Ex: Molds & mushrooms are multicellular (more than one cell) -Yeasts are unicellular (one cell)  
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Protozoa   -Unicellular eukaryotes - superficially animal like, non photosynthetic & usually motile   -May be motile via pseudopods (false feet), cilia or flagella -Causes diseases like malaria & African sleeping sickness  
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Viruses   -are not cells but particles of nucleic acid, Consist of DNA or RNA surrounded by protein coat -Some are enclosed in lipid (fat) envelope   ex: Mad cow disease & scrapie  
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Prions   causes rare neurological diseases ex mad cow disease scrapie in sheep   - chemically & structurally simple like viruses -are infectious proteins -smaller agents that viruses  
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Helminths   parasitic worms; two types Flatworms; ex. tapeworms Roundworms; ex. hookworms   parasitic worms; two types Flatworms; ex. tapeworms  
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parasitic worms; two types   Flatworms; ex. tapeworms Roundworms; ex. hookworms   Flatworms; ex. tapeworms Roundworms; ex. hookworms  
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Robert Hooke   reported that living things were composed of little boxes (cells) after viewing thin slices of cork through crude microscope   reported that living things were composed of little boxes (cells) after viewing thin slices of cork through crude microscope  
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Rudolf Virchow   said cells arise from preexisting cells Cell theory    
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Cell theory   all living things are composed of cells & come from preexisting cells    
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Anton van Leeuwenhoek   described live microorganisms & made detailed drawings of “animalcules” from rainwater, feces & material from teeth scrapings   discovered microorganisms  
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Spontaneous generation   hypothesis that living organisms arise from nonliving matter; “vital force” forms life   Pasteur disproved this theory with germ theory of disease  
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Biogenesis   hypothesis that living organisms arise from preexisting life    
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Louis Pasteur major contribution to science   -principles of vaccination -first vaccines for rabies and anthrax -pasteurization -Unethical clinical trials and some practices   -germ theory of disease  
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Diffraction   Light rays bends when they pass near an opaque object or rays passing through a small opening    
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Resolution   The capacity to perceive 2 adjacent parts of an image as distinct    
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Diffraction   phenomena occur when a wave encounters an obstacle or a slit.    
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Mordant   Compounds or treatments that intensify staining reactions    
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Refraction   Bending that occurs when a ray of light enters an object with a different density at an angle    
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Magnification   enlargement of image    
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culture (o)   The growing of microorganisms, tissue cells, or other living matter in a specially prepared nutrient medium.    
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colony (b)   A clone of cells large enough to be visible on a solid medium    
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Colony   discrete group of organisms, as a collection of bacteria in a culture    
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Pure Culture   A culture that contains only one kind of organism;    
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Pure Culture   one consisting of a single type of microbe derived from a single cell    
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inoculum   is a substance introduced into the body to create or increase the body's resistance or immunity to a disease.   is a substance introduced into the body to create or increase the body's resistance or immunity to a disease.  
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serial dilutions      
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obligate aerobes   Organisms that grow in the presence of O2    
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microaerophile   microorganisms that need lower concentrations of O2 than present in the air    
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facultative anaerobe   Organisms that uses O2 to grow when it is available but can grow with out it    
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areotolerant anaerobe      
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obligate anaerobe   Organisms that grow in the absence of O2    
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types of lens that are used in a microscope      
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differential stain is used for   involve 3 steps: primary staining, destaining, and counter staining    
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special stain      
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Gram stain is used for   a type of differential stain, distinguishing between gram + and -bacteriareflecting differences in their outer surfaces.    
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acid-fast stain is used for   is a difertial stain that colors mycobacteria and actinomycetes.    
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Gram stain steps      
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acid fast stain steps      
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Gram stain results   results red for mycobacteria   all other blue  
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agar are used for      
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petri dishes are used for      
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Defined media   media composed of pure ingredients in carefully measured concentrations dissolved. The exact chemical composition of the medium is known.   Typically, they contain a simple sugar as the carbon and energy source, an inorganic nitrogen source, various mineral salts and if necessary growth factors (purified amino acids, vitamins, purines and pyrimidines  
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Complex media   is one in which the exact contents are not known, can be liquid or solid   is one in which the exact contents are not known, can be liquid or solid  
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Differential media   culture media that enable microbiologist to readily differentiate one organism or group from another   culture media that enable microbiologist to readily differentiate one organism or group from another  
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Selective media   a culture medium that allows a certain organism or group of organisms to grow while inhibiting growth of all other organisms   a culture medium that allows a certain organism or group of organisms to grow while inhibiting growth of all other organisms  
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Contrast   difference in light intensity    
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Archaea   Divided into 3 main groups: Methanogens,halophiles, Extreme thermophiles    
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Algae   -Use photosynthesis for energy -Produce oxygen & their own food -Eaten by other organisms   Important to global ecology but little importance to medicine  
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Protozoa   ex: amoebae, flagellates, ciliates    
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Fungi   -Many are scavengers & breakdown dead organisms Few are pathogenic to humans   pathogenic to plants ex: corn smut, wheat rust, potato blight  
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parasitology   study of Protozoan    
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Viruses are   intracellular parasites   can infect animals, plants & microorganisms  
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Viruses   Viruses are replicated only when they are in living host cell   -Very small, can only be seen with an electron microscope Consist of DNA or RNA surrounded by protein coat  
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Robert Koch   studied and help isolate typhus, dysentery, syphilis, gonorrhea pneumonia, tb   -proved specific microorganisms cause specific disease -used agar to obtain a pure culture  
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Edward Jenner   made vaccine for small pox   made vaccine for small pox  
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germ theory of disease   developed by Robert Koch   microorganisms cause infectious diseases and specific microorganisms cause specific diseases  
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Simple Stains   uses only one dye   uses only one dye  
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Dilute   make (a liquid) thinner or weaker by adding water or another solvent to it.   make (a liquid) thinner or weaker by adding water or another solvent to it.  
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Serial Dilutions   multiple step dilutions   multiple step dilutions  
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Inoculate   The process of introducing an antigenic substance or vaccine into the body to trigger immune response against a specific disease.   The process of introducing an antigenic substance or vaccine into the body to trigger immune response against a specific disease.  
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incubate   have an infectious disease developing inside one before symptoms appear.   have an infectious disease developing inside one before symptoms appear.  
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Enrichment culture   culture media that enable microbiologist to isolate fastidious organisms from samples or specimens and grow them in the lab   culture media that enable microbiologist to isolate fastidious organisms from samples or specimens and grow them in the lab  
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Selective- Differential media   Selective & Differential combined and used to detect salmonella and shigella   Selective & Differential combined and used to detect salmonella and shigella  
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Aerotolerant anaerobe   Aerotololerant anaerobe An organism that live in the presents in O2 but grows best without it   Aerotololerant anaerobe An organism that live in the presents in O2 but grows best without it  
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Strict aerobes   are organisms that require O2   are organisms that require O2  
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Facultative anaerobes   use O2 when available but can do without it   use O2 when available but can do without it  
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Microaerophiles   need low concentrations of O2   need low concentrations of O2  
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what are cellular appendages   structures attached to the envelope and extending beyond the cell   structures attached to the envelope and extending beyond the cell  
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Three commonshapes for bacteria is   spirili- spiral shaped, cocci- round shaped and bascilli- rod shaped   spirili- spiral shaped, cocci- round shaped and bascilli- rod shaped  
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Active Transport   Movement of molecules against concentration requires energy, ATP   movement of a substance against the gradient using energy  
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Pathogens   Disease causing Microorganisms   Disease causing Microorganisms  
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