Lab Final
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| Anaerobic growth (anaerobic jar, candle jar) | No oxygen needed
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| What is Fluid Thioglycollate? What is it used for? What does it contain/purpose? | Rich liquid medium-supports growth of anaerobic and aerobic bacteria. Contains glucose, cysteine and sodium thioglycollate to reduce its oxidation/ reduction potential.
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| What dye is used in anaerobic growth? What are the classifications? | Resazurin (dye) indicator for the presence of oxygen, in presence of oxygen dye becomes pink at the top
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| What is the Kirby-Bauer method used for? What method is used? | Determine the sensitivity or resistance of a bacterium to and antimictobial. Lawn method.
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| How do you measure the zone of inhibition and read it on the zone interpretation charts? | Measure the zone diameters with a metric rule to the nearest whole millimeter. Resistant ( less than = to 8 to 30), Intermediate (9-46), Sensitive (more than = to 13-41)
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| Four microrganisms were worked with; S. aureus, E.coli, P.vulgaris and P, aeruginosa. What genus is most resistant? | Pseudomonas aeruginosa
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| Before testing what color is Glucose Broth? | Red
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| What tube is inside the glucose broth? | Durham tube
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| What indicator dye does glucose broth contain? What is the swing point? | Phenol Red; swing point of 7.
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| What color does a positive glucose test turn? What is being produced if there is a bubble inside the Durham tube? | Yellow (acid production), Gas production (if bubble inside D.tube)
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| What is Methyl red? what is the swing point? | Indicator dye; swing point 5
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| Mixed acid fermenters indication of? | No Krebs Cycle
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| What enzyme is responsible for urea hydrolysis? | Urease
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| What is the color of a positive urease test? | Cerise (bright or deep red color)
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| What color appears for a positive indole test? | A red band appears at the top of the tube
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| What does an indole indicate? (break down into what?) | Tryptophan (tryptophanase)
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| Kligler's iron agar detects the fermentation of what two sugars? | Glucose and Lactose
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| Kligler's iron agar detects the production of what? (testing for) | Hydrogen sulfide resulting from the breakdown of the amino acid cysteine
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| What is Kligler's iron agar useful for? | Differentiation of the gram-negative bacteria
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| What color does a positive Kligler's iron produce? | Dark in bottom of tube (hydrogen sulfide production)
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| What is Spirit Blue agar used to test for? | Lipase (Staphylococcus was positive)
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| What chemical is used in a catalase test? | Hydrogen peroxide
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| What is a characteristic of a positive catalase test? What is being produced? | Bubbles and oxygen gas
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| What is one organism that is catalase positive? | Staphylococcus
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| What color is an original citrate tube? | Green
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| What color is a positive citrate tube? What does that indicate? | Prussian blue; citrate utilization
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| In the VP test, what reagents are used? | Voges and Barrits (aplah too?)
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| What is being produced with the VP test? | Acetoin (alcohol production (butanediol))
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| What color indicates a positive VP test? | Pink or red color
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| What reagent is used in a coagulase test? | Rabbit serum
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| What genus is a coagulase test preformed on to help tell the difference? | Staph aureus (positive-stringy/clumps together) and Staph epidermidis (negative)
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| What is a Staph ID test? | Coagulase test
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| What is m-staphylococcus broth enriched with? | enriched with salt
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| What is one strep ID test? | Hemolysis (beta=clear, gamma=none, apha=green)
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| What is a second Strep ID test? | Antibiotic disks (bacitracin,optim disks, SST-check resistance with Kirby-Bauer test)
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| What media is used for Strep ID tests? | Blood Agar Plate
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| What is a Gram negative rod ID test? | Glucose/ Lactose
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| What media is used in Gram - rod ID tests? | SS agar, EMB agar, McConkeys agar
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| What two organisms are the only lactose negative ones? | Salmonella and Shigella
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| What is an indicator of a lactose negative organism? and what does that mean? | colorless on all media's used; no fermantation
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| What enzyme is starch agar used for? | Amylase
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| What genus is positive for amylase? | Bacillus
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| What chemical is used with starch agar? | Grams iodine
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| What is a result of a postive Amylase test? What is the result of a negative amylase test? | After grams iodine is poured into plate, there will be a clear ring surrounding bacteria. Negative test=all turns black
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| What do pyrimidine dimers do with/ in UV light? | ability to recover from UV light/repairs (skin)
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| What does mpn stand for? (water qualitative) | Most probable Number
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| What is the name of the protein in Skim milk agar? | Casinase
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| What chemical is added to read the starch agar plate? | Gram's Iodine
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| What is the name of the small tube inside the large tube in the glucose broth test? | Durham tube
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| A positive methyl red test tells us which energy pathway is being utilized. Which pathway is it? | Mixed acid fermenters
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| Name a genus that is known lactose negative | Salmonella and Shigella
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| What do we call the first name of a bacterium? | Genus
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