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Micro Lab
Lab Final
| Question | Answer |
|---|---|
| Anaerobic growth (anaerobic jar, candle jar) | No oxygen needed |
| What is Fluid Thioglycollate? What is it used for? What does it contain/purpose? | Rich liquid medium-supports growth of anaerobic and aerobic bacteria. Contains glucose, cysteine and sodium thioglycollate to reduce its oxidation/ reduction potential. |
| What dye is used in anaerobic growth? What are the classifications? | Resazurin (dye) indicator for the presence of oxygen, in presence of oxygen dye becomes pink at the top |
| What is the Kirby-Bauer method used for? What method is used? | Determine the sensitivity or resistance of a bacterium to and antimictobial. Lawn method. |
| How do you measure the zone of inhibition and read it on the zone interpretation charts? | Measure the zone diameters with a metric rule to the nearest whole millimeter. Resistant ( less than = to 8 to 30), Intermediate (9-46), Sensitive (more than = to 13-41) |
| Four microrganisms were worked with; S. aureus, E.coli, P.vulgaris and P, aeruginosa. What genus is most resistant? | Pseudomonas aeruginosa |
| Before testing what color is Glucose Broth? | Red |
| What tube is inside the glucose broth? | Durham tube |
| What indicator dye does glucose broth contain? What is the swing point? | Phenol Red; swing point of 7. |
| What color does a positive glucose test turn? What is being produced if there is a bubble inside the Durham tube? | Yellow (acid production), Gas production (if bubble inside D.tube) |
| What is Methyl red? what is the swing point? | Indicator dye; swing point 5 |
| Mixed acid fermenters indication of? | No Krebs Cycle |
| What enzyme is responsible for urea hydrolysis? | Urease |
| What is the color of a positive urease test? | Cerise (bright or deep red color) |
| What color appears for a positive indole test? | A red band appears at the top of the tube |
| What does an indole indicate? (break down into what?) | Tryptophan (tryptophanase) |
| Kligler's iron agar detects the fermentation of what two sugars? | Glucose and Lactose |
| Kligler's iron agar detects the production of what? (testing for) | Hydrogen sulfide resulting from the breakdown of the amino acid cysteine |
| What is Kligler's iron agar useful for? | Differentiation of the gram-negative bacteria |
| What color does a positive Kligler's iron produce? | Dark in bottom of tube (hydrogen sulfide production) |
| What is Spirit Blue agar used to test for? | Lipase (Staphylococcus was positive) |
| What chemical is used in a catalase test? | Hydrogen peroxide |
| What is a characteristic of a positive catalase test? What is being produced? | Bubbles and oxygen gas |
| What is one organism that is catalase positive? | Staphylococcus |
| What color is an original citrate tube? | Green |
| What color is a positive citrate tube? What does that indicate? | Prussian blue; citrate utilization |
| In the VP test, what reagents are used? | Voges and Barrits (aplah too?) |
| What is being produced with the VP test? | Acetoin (alcohol production (butanediol)) |
| What color indicates a positive VP test? | Pink or red color |
| What reagent is used in a coagulase test? | Rabbit serum |
| What genus is a coagulase test preformed on to help tell the difference? | Staph aureus (positive-stringy/clumps together) and Staph epidermidis (negative) |
| What is a Staph ID test? | Coagulase test |
| What is m-staphylococcus broth enriched with? | enriched with salt |
| What is one strep ID test? | Hemolysis (beta=clear, gamma=none, apha=green) |
| What is a second Strep ID test? | Antibiotic disks (bacitracin,optim disks, SST-check resistance with Kirby-Bauer test) |
| What media is used for Strep ID tests? | Blood Agar Plate |
| What is a Gram negative rod ID test? | Glucose/ Lactose |
| What media is used in Gram - rod ID tests? | SS agar, EMB agar, McConkeys agar |
| What two organisms are the only lactose negative ones? | Salmonella and Shigella |
| What is an indicator of a lactose negative organism? and what does that mean? | colorless on all media's used; no fermantation |
| What enzyme is starch agar used for? | Amylase |
| What genus is positive for amylase? | Bacillus |
| What chemical is used with starch agar? | Grams iodine |
| What is a result of a postive Amylase test? What is the result of a negative amylase test? | After grams iodine is poured into plate, there will be a clear ring surrounding bacteria. Negative test=all turns black |
| What do pyrimidine dimers do with/ in UV light? | ability to recover from UV light/repairs (skin) |
| What does mpn stand for? (water qualitative) | Most probable Number |
| What is the name of the protein in Skim milk agar? | Casinase |
| What chemical is added to read the starch agar plate? | Gram's Iodine |
| What is the name of the small tube inside the large tube in the glucose broth test? | Durham tube |
| A positive methyl red test tells us which energy pathway is being utilized. Which pathway is it? | Mixed acid fermenters |
| Name a genus that is known lactose negative | Salmonella and Shigella |
| What do we call the first name of a bacterium? | Genus |
Created by:
manzanarescalla
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