Lab Assistant Skills (updated)
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| The eyepiece and objectives on a microscope should be cleaned with lens paper after | every use.
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| What concentrates the light on an oil-immersion objective of a microscope. | Oil
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| What diaphragm controls the amount of light that enters a microscope. | iris
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| Any medical laboratory or medical office that performs tests on human specimens is regulated by a federal amendment called the | Clinical Laboratory Improvement Amendment (CLIA).
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| A culture specimen is obtained when a doctor wants to identify the causative agent of | a disease.
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| A slide with a culture specimen is fixed so the organisms will not wash off when | staining solutions are applied.
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| In some states, health science career students are not permitted to perform | any procedure involving obtaining blood.
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| The first drop of blood obtained with a finger puncture is always removed and | not used for a blood test.
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| Microhematocrit capillary tubes are lined with heparin to | keep the blood from clotting.
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| Hemoglobin carries both oxygen | and carbon dioxide.
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| Blood is hemolyzed when | it is clear or transparent.
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| The average value for hemoglobin in adult women is | 12 to 16 grams of hemoglobin per 100 milliilters of blood
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| A blood smear is used to examine the form, structure, and relative number of | erythrocytes, leukocytes, and platelets in blood.
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| Abnormally shaped blood cells can indicate a | disease.
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| Wright’s stain is used to fix a blood smear and to | stain the cells so they are visible.
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| Because certain types of white blood cells increase after specific infections, a different count aids in | making diagnoses.
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| A person’s blood type is determined by | the presence of antigens on the red blood cells.
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| An Rh incompatibility occurs when a pregnant woman is | Rh negative and the developing fetus is Rh positive.
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| An erythrocyte sedimentation rate measures the | distance that red blood cells fall and settle in a specific period of time.
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| Insulin allows glucose to cross cell membranes so the glucose can | be metabolized.
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| Some new glucose meters do not require the use of | reagent strips.
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| Children can be taught to monitor their own | blood sugar levels.
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| For the most accurate results, a urine specimen should be examined within one hour after it is | collected.
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| Reagent strips can be used to determine both the presence of and the amount of | substances in the urine.
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| To check urinary sediments, a fresh, first-voided, | early-morning urine specimen is preferred.
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| Quality-control test chambers are used with most instruments, such as a photometer or hemoglobinometer, to make sure the instrument is | calibrated correctly prior to using it for a blood test.
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| A piece of skin or body tissue can be used | to check for blood type.
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| A faster than normal sedimentation rate signifies that | inflammation and/or cell destruction has taken place.
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| A microscope that uses a dye stain to detect antibodies and specific organisms is a/an | epifluorescence microscope
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| Which of the following is not a test waived by the Clinical Laboratory Improvement Amendment (CLIA)? | differential count
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| The stain that removes the purple color of gentian violet from gram-negative organisms is | ethyl alcohol or acetone-alcohol
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| While performing a Gram’s stain, the safranin solution is applied to the slide for approximately | 30 to 60 seconds
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| An average value for a microhematocrit on a male adult is | 40 to 55 percent
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| The blood cells with a normal count of 5,000 to 10,000 cells per cubic millimeter of blood are | leukocytes
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| An increase in eosophinilscan indicate | parasitic infection
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| If you use 6 drops of Wright’s stain for a blood smear film, how many drops of buffer solution should you use? | 6 drops
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| To perform a quick stain, or three-step method, on a blood smear slide, do all of the following | dip slide into fixative solution for about 1 second
dip slide into two separate staining solutions for 1 second each
repeat dipping procedure four to five times
DO NOT gently rinse the slide with water and blot it dry
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| If a drop of blood reacts to anti-A serum, but does not react to anti-B serum, what type of blood is present? | A
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| The following substances is frequently present in the urine of a person with diabetes mellitus? | ketones or acetones
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| A reagent strip that checks for the presence of glucose is a | Diastix
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| An ammonia odor in a urine specimen usually indicates | old specimen
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| The test that measures the average blood sugar level for the previous two to three months is the | glycohemoglobin test
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| The presence of pus in the urine is | pyuria
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| Most states require that a blood and/or urine test is performed on all newborns to check for the presence of the amino acid or protein called | phenylalanine
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| Blood test that measures the volume of packed red blood cells | Hematocrit
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| Substance that prevents clotting of the blood | Anticoagulant
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| Blood test that measures the oxygen-carrying capacity of blood | Hemoglobin
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| Blood test that determines the number of red blood cells | Erythrocyte count
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| Blood test that determines the percentage of each type of white blood cell | Differential count
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| Destruction of red blood cells that releases hemoglobin into the solution that surrounds the cells | Hemolysis
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| Blood test done prior to a transfusion of blood | Typing and crossmatch
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| A culture specimen is placed on a slide | Direct smear
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| Organisms on a culture plate avoid growing near an antibiotic disc | Sensitive
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| Weight of a substance compared with the weight of distilled water | Specific gravity
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| Less than normal amounts of urine | Oliguria
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| A microscopic test done to examine the solid materials suspended in the urine | Urinary sediments
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| Presence of blood in the urine | Hematuria
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| A float with a calibrated stem used to measure specific gravity | Urinometer
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| What is the total magnification of a microscope if the eyepiece is 20X and the objective is 40X? | 800X
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| Specific standard precautions that must be observed while handling cultures. | 1. wash hands frequently, 2. wear gloves, 3. wear lab coats or aprons, 4. wear a mask and protective eyewear if splashing can occur, 5. place all waste in an infectious waste bag, 6. clean any contaminated areas with a disinfectant solution
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| Major diseases that can be transmitted by blood. | 1. hepatitis B (HBV), 2. hepatitis C (HBC), 3. acquired immune deficiency syndrome (AIDS)
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| Calculate the correct hematocrit reading if one tube reads 33 percent and a second tube reads 36 percent. | 34.5 percent (33 + 36 = 99 ÷ 2 = 34.5)
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| If a drop of blood reacts to anti-Rh serum, but does not react to either anti-A or anti-B serum, what is the blood type? | O+
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| Advantages to checking blood glucose levels instead of urine glucose levels. | 1. increased accuracy over urine, 2. ability to detect both low and high glucose levels, 3. better regulation of insulin dosage, 4. flexibility with regard to time of the test, 5. improved control over diabetes
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| What is a normal level for fasting blood sugar? | 70 to 110 milligrams per deciliter of blood
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| what is examined during the following area of testing in a urinalysis? physical testing | color, odor, transparency, specific gravity
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| what is examined during the following area of testing in a urinalysis? chemical testing |
pH, protein, glucose, ketone, bilirubin, urobilinogen, and blood
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| what is examined during the following area of testing in a urinalysis? microscopic testing | formed elements in the urine such as cells, casts, crystals, and amorphous deposits
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| What is the normal range for specific gravity of urine? | 1.005 to 1.030
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| A Unopette is a disposable, self-filling, blood-diluting | pipette unit.
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| A low white blood cell count indicates | leukopenia
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| Wintrobe or Westergren are two main methods used to perform this test | Erythrocyte sedimentation rate
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| Physical, chemical, and microscopic examination of urine is called | Urinalysis
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| Points that must be checked before performing a skin puncture to obtain blood | no edema, no calluses, no scars, no rash, no sores, skin is warm to touch, skin is not cyanotic
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| When performing a skin puncture avoid | using the thumb, index finger or pinkie finger
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| A sample of microorganisms or tissue cells taken from an area of the body for examination | culture specimen
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| A special laboratory dish used to provide nourishment for growth of organisms | agar plate
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| Test that test for antibodies in the blood prior to a transfusion | antibody screen
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| Substance that causes the body to produce antibodies; may be introduced into the body or formed within the body | antigen
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| Without urine; producing no urine | anuria
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| Erythrocyte | red blood cells (RBC)
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| Blood test that measures blood serum levels of glucose (sugar) after a person has had nothing by mouth for a period of time | fasting blood sugar (FBS)
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| The most common type of sugar in the body | glucose
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| A diagnostic test that evaluates how well a person metabolizes a calculated amount of glucose | glucose tolerance test
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| A blood test that measures the amount of glucose that attaches to hemoglobin on red blood cells to determine the average blood-sugar levels for the previous 2 to 3 months | glycohemoglobin test
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| Technique of staining organisms to identify specific types of bacteria present | gram's stain
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| Blood test that measures the percentage of red blood cells per a given unit of blood | hematocrit (Hct)
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| The iron-containing protein of the red blood cells; serves to carry oxygen from the lungs to the tissues | hemoglobin (Hgb)
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| Presence of sugar in the blood | hyperglycemia
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| Low blood sugar | hypoglycemia
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| White blood cells are called | leukocytes (WBC)
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| Instrument used to magnify or enlarge objects for viewing | microscope
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| Special test strip containing chemical substances that react to the presence of certain substances in the urine or blood | reagent strips
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| An instrument used to measure the specific gravity of urine | refactometer
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| Organisms that remain unaffected by harmful substances in the environment | resistant
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| Susceptible to a substance; organisms that are affected by an antibiotic in a culture and sensitivity study | sensitivity
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| A small puncture made in the skin to obtain capillary blood is call a | skin puncture
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| A determination of blood types and antigens prior to a blood transfusion | typing and crossmatch
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| Examination of urine by way of physical, chemical, or microscopic testing | urinalysis
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| Solid materials suspended in urine | urinary sediments
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| Calibrated device used to measure the specific gravity of urine | urinometer
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| Inserting a needle into a vein is called a | venipuncture
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