Questions about "Pour Plate"
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| A freshly poured plate should be rotated carefully in a ___________. | figure-eight
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| What is the predominant shape of subsurface colonies? | Lens shape.
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| Which method of separating organisms, streak plate or pour plating, seems to achieve the best separation? | Pour plating.
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| Give 2 reasons why the nutrient agar must be cooled to 45°C before inoculating and pouring: | Lower temperature results in less condensation.
Lower temperature won't kill the organism.
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| The name of the instrument use to help count colonies on plates is the ________ ________ ________. | Quebec colony counter
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| This technique is said to be roughly quantitative because the loop used contains only approximately ________ ml. | 0.01
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| If your tubes solidify after inoculation but before you pour them into plates, what happens to the bacteria in the tubes when you now remelt the agar? | Bacteria are killed at remelting temperatures.
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| Give one reason why you should avoid slopping agar up on the cover while mixing the sample. | Slopping agar can be a source of contamination of the environment around the plate. Can also block line of sight, resulting in an invalid colony count.
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| When you incubate these plates, the cover should be ________. | down
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| When pouring an agar plate, remove the cover and lay it on the bench. | Bad aseptic technique.
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| Hold the cover up just enough to admit the neck of the flask. | Good aseptic technique.
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| Flame the neck of the agar flask before pouring a plate. | Good aseptic technique.
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| As soon as the tube is inoculated in this exercise, remove the cap and immediately pour the plate. | Bad aseptic technique.
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| Heat the lip of the tube so the agar sizzles as it is being poured into the plate. | Bad aseptic technique.
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| Incubate your plates with the cover up. | Bad aseptic technique.
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| After streaking a plate with a pure culture, lay the loop down on the bench. | Bad aseptic technique.
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| Carefully remove the cap on a tube, and without setting it down, make your transfer. | Good aseptic technique.
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| Flame your inoculating loop before and after making your transfer. | Good aseptic technique.
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| While attempting to remove a tube cap, your loop touches the sleeve of your lab coat. | Bad aseptic technique.
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Created by:
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