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MicrobiologyLab9

Questions about "Pour Plate"

QuestionAnswer
A freshly poured plate should be rotated carefully in a ___________. figure-eight
What is the predominant shape of subsurface colonies? Lens shape.
Which method of separating organisms, streak plate or pour plating, seems to achieve the best separation? Pour plating.
Give 2 reasons why the nutrient agar must be cooled to 45°C before inoculating and pouring: Lower temperature results in less condensation. Lower temperature won't kill the organism.
The name of the instrument use to help count colonies on plates is the ________ ________ ________. Quebec colony counter
This technique is said to be roughly quantitative because the loop used contains only approximately ________ ml. 0.01
If your tubes solidify after inoculation but before you pour them into plates, what happens to the bacteria in the tubes when you now remelt the agar? Bacteria are killed at remelting temperatures.
Give one reason why you should avoid slopping agar up on the cover while mixing the sample. Slopping agar can be a source of contamination of the environment around the plate. Can also block line of sight, resulting in an invalid colony count.
When you incubate these plates, the cover should be ________. down
When pouring an agar plate, remove the cover and lay it on the bench. Bad aseptic technique.
Hold the cover up just enough to admit the neck of the flask. Good aseptic technique.
Flame the neck of the agar flask before pouring a plate. Good aseptic technique.
As soon as the tube is inoculated in this exercise, remove the cap and immediately pour the plate. Bad aseptic technique.
Heat the lip of the tube so the agar sizzles as it is being poured into the plate. Bad aseptic technique.
Incubate your plates with the cover up. Bad aseptic technique.
After streaking a plate with a pure culture, lay the loop down on the bench. Bad aseptic technique.
Carefully remove the cap on a tube, and without setting it down, make your transfer. Good aseptic technique.
Flame your inoculating loop before and after making your transfer. Good aseptic technique.
While attempting to remove a tube cap, your loop touches the sleeve of your lab coat. Bad aseptic technique.
Created by: NeuroHeart
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