Microbiology
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
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Aliquotes | small samples of the culture
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Anabolism | use of energy and building blocks to produce new cellular materials, such as macromolecules
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Anaerobic respiration | bacteria in the abswence of oxygen--utilize nitrate/sulfate as the final electron acceptor
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Autolytic | bacteria that produce enzymes that cause their own lysis or break down
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Binary fission | (cell division) bacterial cells grow by dividing and forming 2 progeny cells that are equal to the original cell
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Catabolism | breakdown of complex materials into building blocks/energy--cells use for synthesis--making more cellular chemicals
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Cell division | (binary fission) bacterial cells grow by dividing and forming two progeny cells that are equal to the original cell
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Chemosynthetic metabolism | bacteria can use salts found in rock as an energy source--require little to grow--generally autotrophs--make organic chemicals from carbon dioxide, chemicals dissolved in water, energy from rocks
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Colony forming unit | CFU--arises from single diluted cell--represents viable bacterial cells--those cells that could grow and form a colony
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Death phase | cells are not receiving sufficient energy or nutrients to maintain viability
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Decline phase | (death phase) cells not receiving sufficient energy or nutrients to maintain viability
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Denature | enzymes unfold when they are boiled and are no longer active
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Dilution | used to determine bacterial cell numbers--dilutions performed at each time point--aliquots spread on petri dishes--incubate--count how many colonies grow--resulting colony count multiplied by dilution factor to know how many bacteria in original sample
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Electron transport chain | consists of a series of carriers in the membrane--carriers use electron-derived energy to pump protons out of cell--creates proton gradient with higher concentration on outside--protons flow back into cell through proteinaceous carrier ATP
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Exponential growth | when bacteria double every constant period of time
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Feedback inhibition | (feedback regulation) mechanisms of gene expression control--bacteria can preclude the need to make thousands of temp. unneeded proteins that would otherwise reduce growth efficiency dramatically
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Fermentation | process of incomplete catabolism of sugars to produce alcohol in wines
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Generation | division or doubling of bacteria
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Glycolysis | in cells utilizing glucose, the glucose is transported to the inside and oxidized
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Krebs cycle | (TCA cycle--tricarboxylic acid cycle) oxygen is used to degrade carbon completely to carbon dioxide, water, ATP, energy-containing electrons--passed on to te electron transport chain
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Lag phase | cells adapting to rich supply of nutrients in preparation for growth
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Log phase | cells start to multiply by doubling every perhaps 30 minutes
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Metabolism | all chemical processes that occur in a cell
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Oxidative phosphorylation | transfer of electrons to oxygen coupled to the synthesis of ATP
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Product | sucrose in reaction
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Pyruvate | six-carbon sugar is converted into two three-carbon acids, called pyruvic acid
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Reactants | (substrates) glucose and fructose in reaction
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Reaction | one enzyme can use the sugars glucose and fructose and combine them to disaccharide sucrose--common table sugar
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Respiration | passage of electrons to oxygen to produce water
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Stationary phase | cells maintain cell number but not able to produce new cells--dying and producing new cells at about the same rate
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Substrates | (reactants) glucose and fructose in reaction
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Total cell count | resulting number of dilutions
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Turbidity | (blank)
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Viable counts | (blank)
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Created by:
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