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Microbiology

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
Aliquotes   small samples of the culture  
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Anabolism   use of energy and building blocks to produce new cellular materials, such as macromolecules  
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Anaerobic respiration   bacteria in the abswence of oxygen--utilize nitrate/sulfate as the final electron acceptor  
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Autolytic   bacteria that produce enzymes that cause their own lysis or break down  
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Binary fission   (cell division) bacterial cells grow by dividing and forming 2 progeny cells that are equal to the original cell  
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Catabolism   breakdown of complex materials into building blocks/energy--cells use for synthesis--making more cellular chemicals  
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Cell division   (binary fission) bacterial cells grow by dividing and forming two progeny cells that are equal to the original cell  
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Chemosynthetic metabolism   bacteria can use salts found in rock as an energy source--require little to grow--generally autotrophs--make organic chemicals from carbon dioxide, chemicals dissolved in water, energy from rocks  
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Colony forming unit   CFU--arises from single diluted cell--represents viable bacterial cells--those cells that could grow and form a colony  
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Death phase   cells are not receiving sufficient energy or nutrients to maintain viability  
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Decline phase   (death phase) cells not receiving sufficient energy or nutrients to maintain viability  
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Denature   enzymes unfold when they are boiled and are no longer active  
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Dilution   used to determine bacterial cell numbers--dilutions performed at each time point--aliquots spread on petri dishes--incubate--count how many colonies grow--resulting colony count multiplied by dilution factor to know how many bacteria in original sample  
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Electron transport chain   consists of a series of carriers in the membrane--carriers use electron-derived energy to pump protons out of cell--creates proton gradient with higher concentration on outside--protons flow back into cell through proteinaceous carrier ATP  
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Exponential growth   when bacteria double every constant period of time  
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Feedback inhibition   (feedback regulation) mechanisms of gene expression control--bacteria can preclude the need to make thousands of temp. unneeded proteins that would otherwise reduce growth efficiency dramatically  
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Fermentation   process of incomplete catabolism of sugars to produce alcohol in wines  
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Generation   division or doubling of bacteria  
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Glycolysis   in cells utilizing glucose, the glucose is transported to the inside and oxidized  
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Krebs cycle   (TCA cycle--tricarboxylic acid cycle) oxygen is used to degrade carbon completely to carbon dioxide, water, ATP, energy-containing electrons--passed on to te electron transport chain  
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Lag phase   cells adapting to rich supply of nutrients in preparation for growth  
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Log phase   cells start to multiply by doubling every perhaps 30 minutes  
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Metabolism   all chemical processes that occur in a cell  
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Oxidative phosphorylation   transfer of electrons to oxygen coupled to the synthesis of ATP  
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Product   sucrose in reaction  
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Pyruvate   six-carbon sugar is converted into two three-carbon acids, called pyruvic acid  
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Reactants   (substrates) glucose and fructose in reaction  
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Reaction   one enzyme can use the sugars glucose and fructose and combine them to disaccharide sucrose--common table sugar  
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Respiration   passage of electrons to oxygen to produce water  
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Stationary phase   cells maintain cell number but not able to produce new cells--dying and producing new cells at about the same rate  
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Substrates   (reactants) glucose and fructose in reaction  
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Total cell count   resulting number of dilutions  
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Turbidity   (blank)  
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Viable counts   (blank)  
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