Lab Quiz 5-8
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
|
|
||||
|---|---|---|---|---|---|
| What are the three different carbs tested for in the Phenol Red Broth test? | Glucose, Lactose, and Sucrose.
🗑
|
||||
| How to interpret the results of a Phenol Red Broth test (card 1 of 2) | Yellow broth with bubble in tube = Fermentation with acid and gad end products
Yellow broth with no bubble in tube = Fermentation with acid end products but no gas
🗑
|
||||
| How to interpret the results of a Phenol Red Broth test (card 2 of 2) | Red Broth with no bubble in tube = No fermentation
Pink Broth with no bubble in tube = Degradation of peptone, alkaline end products
🗑
|
||||
| Which enzymes must microbes produce to break down either lactose or sucrose? | To break down lactose - B-galctosidase
To break down sucrose - Sucrase
🗑
|
||||
| What is Methyl Red Used for? | Detecting whether or not an organism can conduct mixed acid fermentation
🗑
|
||||
| What is Voges-Proskauer used for? | Detecting organisms that utilize butylene glycol to produce acetoin
🗑
|
||||
| What are some examples of mixed acid fermentation byproducts? | Lactic Acid
Acetic Acid
Formic Acid
🗑
|
||||
| What reagents used to produce a color change in a methyl red test? | Methyl Red
🗑
|
||||
| What reagents are used to produce a color change in a Voges-Proskauer test? | Barritt’s A and Barritt’s B reagent
🗑
|
||||
| How do you interpret the results of a methyl red test? | Red result means positive for mixed acid fermentation, no color change means no mixed acid fermentation
🗑
|
||||
| How do you interpret the results of a Voges-Proskauer test? | Red result means acetoin production, no color change means no acetoin has been produced
🗑
|
||||
| What is the purpose of a citrate test? | To find out whether or not an organism can utilize citrate as a carbon source
🗑
|
||||
| What reagent is used in a citrate test and what are the byproducts? | Reagent - Sodium citrate, has byproducts oxaloacetate & pyruvate.
🗑
|
||||
| What is an oxidase test used for? | Oxidase is used to identify organisms that produce the enzyme cytochrome oxidase.
🗑
|
||||
| What is a catalse test used for? | Catalase is used to identify cells that can break down hydrogen peroxide into H2O and O2.
🗑
|
||||
| In a positive oxidase test what enzymes are produced? | A positive oxidase test produces cytochrome oxidase.
🗑
|
||||
| In a positive catalase taste what enzymes are produced? | A positive catalase test produces catalase.
🗑
|
||||
| What is the cytochrome oxidase enzyme used for? | Cytochrome oxidase is used in the electron transport chain as a donor transfer electrons to oxygen
🗑
|
||||
| What is the catalase enzyme used for? | Catalase is used to get rid of peroxide in an O2 environment
🗑
|
||||
| What does a positive catalase test look like? | A positive catalase test produces bubbles because of the evolution of O2 gas.
🗑
|
||||
| How is a positive oxidase reaction determined? | A positive oxidase reaction will turn blue or purple within 15 seconds
🗑
|
||||
| How is a positive catalase reaction determined? | A positive catalase reaction is determined by bubbling.
🗑
|
||||
| What does a positive oxidase test look like? | An oxidase positive will turn violet to purple immediately or within 10 to 30 seconds. Delayed reactions should be ignored.
🗑
|
Review the information in the table. When you are ready to quiz yourself you can hide individual columns or the entire table. Then you can click on the empty cells to reveal the answer. Try to recall what will be displayed before clicking the empty cell.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
To hide a column, click on the column name.
To hide the entire table, click on the "Hide All" button.
You may also shuffle the rows of the table by clicking on the "Shuffle" button.
Or sort by any of the columns using the down arrow next to any column heading.
If you know all the data on any row, you can temporarily remove it by tapping the trash can to the right of the row.
Embed Code - If you would like this activity on your web page, copy the script below and paste it into your web page.
Normal Size Small Size show me how
Normal Size Small Size show me how
Created by:
skline1982
Popular Biology sets