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Test 1

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
Kinds of cells   Eukaryotes prokaryotes  
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DNA in Prokaryotes   DNA is not enclosed in nuclear membrane, one circular chromosome, not associated in with histones  
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Prokaryotic Cells   no membrane enclosed organelles  
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DNA Eukaryotes   DNA encolsed in nuclear membrane, multiple chromosomes, associated w/ histones and non histones  
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Eukaryotic cells   golgi complex, mitochondria, lysosomes  
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Prokaryotes   include bacteria and archaea  
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Species of Bacteria are Differeniated by   Morphology, chemocal composition, nutritional requirements, biochemical activities, source of energy  
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Basic Shapes   Bacillus, cocci, spiral,  
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Bacillus   Rod shape  
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Coccus   spherical  
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Spiral   Spirillum, Vibrio(comma shaped), spirochetes  
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Sarcinae   Coccus- remain in groups of 8  
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Streptococci   remain attached in chainlike pattern  
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Staphylococci   fomr grapelike structures  
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Spirochetes   helical and flexible  
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Unsual bacterial shapes   stella and haloarcula  
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Shape determination   heredity  
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Monomorphic   maintain a single shape  
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Pleomorphic   can have more than one gentically controlles shape  
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Pleomorphic bacteria   Rhizobium, corynebacterium  
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Prokaryotic cell layers   outer membrane, cell wall, capsule, plasma membrane, nucleoid region, ribosomes, pilus, flagellum  
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Glycocalyx   Sugar coat on surface of many cells, made inside and secreted outside  
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Capsule   organized and firmly attached glycocalyx  
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Slime layer   unorganized and loosly attached glycocalyx  
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Purpose of capsule   provides protection against phagocytosis, allow bacteria to attach to surfaces, source of nutrition, protects from dehydration  
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Flagella   Motility  
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Arrangements of flagella   monotrichous, amphitrichous, lophotrichous, peritrichous  
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Monotrichous   single polar flagellum  
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amphitrichous   tuft of flagella on both ends  
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lophotrichous   2 or more flagella at one pole  
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peritrichous   flagella distributed all over cell  
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Parts of flagella   Filament, hook, basal body  
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Filament   long outermost part, constant diameter, composed of flagellin  
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flagellin   globular protein, arranged in intertwining chains, forming,helix, around hollow core  
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Hook   filament attached to it, wider, made of different protein  
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basal body   small central rod inserted into a series of rings  
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gram negative basal body   outer ring anchored to various portions of cells; inner ring attachedto plasma membrane  
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gram positive basal body   one pair of rings, only one paired attached to plasma membrane  
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Run   flagella movement is in one direction  
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tumbles   abrupt random changes in direction  
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taxis   movement torward or awayfrom a stimulus  
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Positive Taxis   movement torward an attractant  
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Negative Taxis   movement away from repellent  
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Axial Filament location   Found on spriochetes and also called endoflagella  
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Axial filament   made of bundles of fibrils beneath an outer sheath and spiral around cell  
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Fimbrae & Pili   found in gm neg bacteria, shorter and thinner than flagella  
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composition of fimbrae & Pili   made of protein called pilin  
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Fimbrae   enables a cell to adhere to surfaces, if not present no colonizationa  
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Pili   Sex Pili, 1 or 2 found on cells; join cells for transfer of DNA from one cell to another  
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the cell wall   point of anchorage for flagella  
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pseudomurein   archaea cell wall - modification of muramic acid  
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Peptidoglycan composed of   NAG and NAM which make carbohydrate backbone  
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rows are linked by   polypeptides that always include tetrapeptide side chains of 4 amino acids attached to NAM  
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Penicillin   interferes with final linking of peptidoglycan rowsby peptide crossing bridge  
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Gram positve cell wall   many layers of PTG, contain techoic acid(Lipo/Wall)  
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Lipotechoic   Linked to plasma membrane  
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wall techoic   linked to PTG  
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Gram Negative cell wall   one or few layers of peptidoglycans, no techoic acid  
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PTG   present in periplasm  
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Lipid A   endotoxin causes fever and shock  
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O Polysaccahride   is antigenic and ised for ID  
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lysozymes   target cell wall- cuts the bond btwn sugars  
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protoplasts   help rebuild cell wall  
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Osmotic lysis   Destruction of protoplast and spheroplasts  
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Passive process   substance moves from higher concentration to lower concentration w/o energy  
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Active process   substance moves from area of lower concentration to higher concentration w/ energy  
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hypotonic solution   cell with weak cell wall may burst  
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hypertonic   bacterial cells shrink and collapse  
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group translocation   only prokaryotes- special type of active transport  
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Group translocation energy   Phosphoenol Pyruvic Acid (PEP)  
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Plasmids   small circular double stranded DNA which are extra- chromosomal- carry genes for abx resistance.  
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70S   prokaryotic ribosomes composed of 50S and 30S  
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50S   large subunit attacked by erythromycin and cloramphenicol  
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30S   smaller subunit attacked by mycin's  
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