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Microbiology 2

Quiz yourself by thinking what should be in each of the black spaces below before clicking on it to display the answer.
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Question
Answer
prokaryotes include   bacteria and archaea  
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bacteria examples include   cyanobacteria, heterotrophic bacteria  
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archaea exammples include   halophiles and thermophiles  
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pathogens include   bacteria and eukaryota  
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ukaryotes include   basal protists, flagellates, fungi, rhodophytes, alveolates,chromists, plants and animals  
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prokaryotes have a...with...   primitive nucleus...no nuclear membrane  
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archaea (which is a..) is similar to...and lives in...such as...   domain...bacteria...harsh environments...hot springs, salt lakes, soils and marshlands  
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archaea are particularly numerous in the   oceans  
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there are no known   pathogens in archaea  
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bacteria (which is a...) do include...   domain...pathogens  
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eukaryotes (which is a...) have a...with...   domain...true nucleus...nuclear membrane  
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eukaryotes are things like...which can include...   humans, spiders, flies, plants, molds, yeasts and parasites...pathogens  
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bacteria and archaea evolved...and are...   3-4 billion years ago...unicellular  
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eukaryote: protista evolved...and are...   1.5 bil years ago...unicellular  
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eukaryote: fungi evolved...and are...   1 bil years ago...multi or unicellular  
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eukaryote: animalia evolved...and are...   700 mil years ago...multicellular  
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eukaryote: plantae evolved...and are...   500 mil years ago...multicellular  
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ribosomes do...and are they present or not in pro/eu?   protein synthesis...present in both  
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cytoskeleton creates the...and provides...   shape in prokaryotes...support, cytoplasmic streaming and endocytosis in eukaryotes  
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cytoskeleton is present in some...and in all...   prokaryotes...eukaryotes  
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centrioles appear to play a role in...and...and...   mitosis...cytokinesis...flagella and cilia formation in animal cells  
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centrioles are...in prokaryotes and...   absent in all....present in animal eukaryots  
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membranous organelles are   absent in all prokaryotes  
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nucleus is the...of the cell and is...in eukaryotes   control center...present in all  
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ER does...and is...in eukaryotes   transportation and lipid synthesis...present in all  
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golgi bodies do...and are... eukaryotes   exocytosis and secretion...present in some  
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lysosomes do two things...and are...eukaryotes   breakdown of nutrients, self-destruction of damaged or aged cells...present in some  
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peroxisomes do...and are...eukaryotes   neutralization of toxins...present in some  
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vacuoles do...and are...eukaryotes   storage...present in some  
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vesicles do...and are...   transport, digestion and storage...present in all eukaryotes  
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mitochondria do..and are...   aerobic ATP production...present in most eukaryotes  
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chloroplasts do...and are present in...   photosynthesis...eukaryotic plants, algae and some protozoa  
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prokaryotes have only   one copy of DNA (haploid)  
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the most important organelle for differentiating between eukaryote and prokaryotes is the   nucleus  
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all eukaryotes have a nucleus except for   rbcs  
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eukaryotes have organelles for...but prokaryotes...   specific functions...have no organelles  
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components of eukaryotic cells whicch would not be considered organelles include   microtubules/filaments and chromosomes  
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microtubules and microfilaments make up the...and perform   cytoskeleton structure...movement by forming pseudopoda  
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chromosomes are...and do...   organized...packaging of DNA involving histone proteins  
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prokaryotic cells range from...and eukaryotic cells range from...   .2-2micrometers...10-100  
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prokaryotic flagella consist of   two protein building blocks  
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eukaryotic flagella are...and consist of..   complex...multiple microtubules  
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prokaryotic glycocalyx are...and...   present...are a capsule or slime layer  
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eukaryotic glycocalx is..that lack...   present in some cells...a cell wall  
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prokaryotic plasma membrane contains no..and generally lacks...   carbohydrates...sterols  
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the plasma membrane of a eukaryotic cell has...present   sterols and carbohydrates that serve as receptors  
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prokaryotic cytoplasm has   no cytoskeleton or cytoplasmic steraming  
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eukaryotic cytoplasm has   cytoskeleton and cytoplasmic streaming  
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ribosomes of prokaryotic cells are   smaller in size (70s) compared to eukaryotic (80s)  
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prokaryotes go through...for cell division   binary fission  
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eukaryotic cells go through...for cell division   mitosis  
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prokaryotes have   single circular chromosome that lacks histones  
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eukaryotes have   multip[le linear chromosomes, with histone arrangement  
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prokaryotes and sexual reproduction   no meiosis, transfer of DNA fragments only  
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eukaryotes and sexual reproduction   involves meiosis  
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cilia are only in...and the motion is in...   eukaryotes...one plane(beating  
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flagella are in...but they have...   both pro and eu...different structures  
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flagella motion is...or...   rotational in pro...undulation in eukaryotes  
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flagella are used for...and direction is determined by...   locomotion...phototaxis or chemotaxis  
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phototaxis means   moving toward light  
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chemotaxis means   moving toward chemical attractant  
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eukaryotic flagella are   internal, entirely surrounded by cytoplasmic membrane  
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eukaryotic flagella are only found on...and their action is..   one pole of cell...rhythmic undulation which pushes or pulls the cell  
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prokaryotic flagella are...and several...   imbedded in cell envelope...configurations existt  
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prokaryotic flagella action is   rotational, cells move by "run and tumble"  
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a run is when...and tumble is when..   flagella propel prokaryote in a CCW direction..it spins in a CW direction  
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prokaryotic flagella have 4 configurations   monotrichous, lophotrichous (polar), amphitrichous (bipolar) and peritrichous  
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eukaryotic and prokaryotic flagella function...   differently in their mechanism of propulsion  
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eukaryotic flagella   whip back and forth (undulate)  
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prokaryotic flagella...and involves the process of...   rotate...chemotaxis  
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flagellin contaqin many copies of   a single protein that make up the filament  
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flagellin also contain a...that is a..   basal body...protein  
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the prokaryotic flagellin penetrates through the...where it is anchored by the...   cell wall and plasma membrane...basal boedy  
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just outside the cell is the...and then the...   hook...filament (made of protein that is specific to that type of cell so you can stereotype them)  
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bacterial spirochetes have   axial fibrils in a sheath called axial filaments  
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bacterial spirochetes are   helical/spiral-shaped bacteria  
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bacterial spirochetes move by...which allows it to...   corkscrewing..burrow into cells  
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eukaryotic flagella and cilia do not...and their action is ina...   pierce the cell membrane...beating fashion (not rotary as in prokaryotes)  
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cilia are...present only on...   short hair like structures...eukaryotes  
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cilia extend from   cell and provide locomotion  
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functions of cilia include   locomotion in unicellular organisms and prevention of lung damage in resp tract by pushing out microbes/debris  
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motile wbcs move by use of...   pseudopoda  
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wbcs are motile but they..by means of the...   crawl...microfilaments and microtubules forming the pseudopods  
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pseudopods help perform   phagocytosis  
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the ribosomes are a   key difference between pro and eukaryotes (think size)  
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70s vs 80s: s is the   sedimentation factor (70 for pro, 80 for euk)  
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ribosomal RNA and many proteins differ in   structure and between eukaryotes and prokaryotes  
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differing ribosomal RNA is very imp when it comes to   treatment of bacterial diseases by targeting something that is different from oour cells  
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prokaryotic cell wall is...and...   usually present...chemically complex (typical bacterial cell wall includes peptidoglycan)  
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eukaryotic cell wall, when present is...   chemically simple  
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cytosol and inclusions are present in   all pro and euk  
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endospores are present in...and...   some pros and absent in all euks  
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bacterial morphology/shapes include   bacillus (oval), coccus (round), and spirillum (spiral)  
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bacillus example   bacillus anthracis (anthrax)  
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coccus example   staphylococcus aureus or MRSA infection  
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spirochete example   treponema pallidum or syphillis  
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aggregations of cocci can either be   streptococci (chain), diplococci (pair) or staphylococci (clump)  
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streptococci example   streptococcus pyrogenes  
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diplococci (stop at one cell division) example   neisseria gonorrhea  
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staphylococci example   staphylococcus aureus  
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cytosol is also called...and is...   cytoplasmic matrix...liquid found inside cells which is complex mixture of substances dissolved in water  
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in prokayrotes, most...take place in the cytosol   chemical reactions of metabolism  
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cell membrane/cytoplasmic membrane/plasma membrane: like all biological membranes, the bacterial cytoplasmic membrane is composed of   phospholipid and protein molecules  
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the phospholipid has a...and a...   hydrophobic part...hydrophilic part  
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prokaryotic membranes   lack sterols  
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bacterial cytoplasmic membrane does   diffusion, active transport, energy production and motility  
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diffusion can either be   passive or facilitated diffsuion through a channel protein  
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energy production involves the   electron transport system for bacteria with aerobic and anaerobic respiration (mitochondria)  
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bacterial cytoplasmic membrane does motility and involves   the bases of bacterial flagella residing within cell membrane  
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diffusion happens through the   phospholipid bilayer  
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facilitated diffusion happens through a   nonspecific channel protein  
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facilitated diffusion through a...leads to...which leads to...   permease specific for one chemical...binding of substrate...shape change in channel protein  
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osmosis is the...through a..   diffusion of water...specific channel protein or through the phospholipid bilayer  
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example of active transport would be   generation of ap roton gradient across membrane for energy production  
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active transport uses...and the same principle can be used to pump...   atp...antibiotic drugs out in some mechanisms of drug resistance  
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2 major categories based on gram stain   gram positive or negative depending on structure of cell wall  
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pili are also called...and they do not do...   fimbriae...movement  
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pili are   hair like structures protruding from the cell envelope  
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type 1 fimbriae aare...and are used to...which is a...   fuzzy..attach to target tissues...virulence factor  
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virulence factors allow   organism to colonize and become pathogenic  
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type 2 fimbriae are also called...and are present in...   sex pilus...only gram negative bacteria  
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type 2 fimbriae are used for   genetic exchange  
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type 2 fimbriae inject   plasmin w/ extra dna to new bacterium  
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a capsule is a   discrete mucoid layer of polysaccharides deposited outside the cell wall of bacteria  
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sometimes bacterial cells are embedded more...in a...called a..   randomly...polysaccharide matrix...slime layer or biofilm  
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capsules have several functions including   mediating adherence of cells to surfaces (biofilm - dental plaque)and they protect bacterial cells from engulfment by white blood cells (phagocytosis)  
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many pathogenic bacteria are   encapsulated in the slime layer which is a virulence factor  
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an example of biofilm construction in nature is the formation of...mediated by the...   dental plaque...oral bacterium streptococcus mutans  
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bacteria adhere specifically to the...   pellicle of the tooth by means of a protein on the cell surface  
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the bacteria are able to...into..   cleave sucrose (provided by diet)...glucose plus fructose  
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fructose is...as an...   fermented...energy source for bacterial growth  
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glucose is...into an...that binds the...   polymerized..extracellular dextran polymer...bacteria to the enamel and forms a biofilm some 300-500 cells in thickness  
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biofilm cements the   bacteria to the tooth enamel and becomes the matrix of dental plaque  
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the dextran slime can be...for use as a...   depolymerized to glucose...carbon source  
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depolymeriing dextran slime results in production of..within the biofilm that...   lactic acid...decalcifies the enamel and leads to dental caries or bacterial infection of the tooth  
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