Microbiology 2
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prokaryotes include | bacteria and archaea
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bacteria examples include | cyanobacteria, heterotrophic bacteria
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archaea exammples include | halophiles and thermophiles
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pathogens include | bacteria and eukaryota
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ukaryotes include | basal protists, flagellates, fungi, rhodophytes, alveolates,chromists, plants and animals
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prokaryotes have a...with... | primitive nucleus...no nuclear membrane
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archaea (which is a..) is similar to...and lives in...such as... | domain...bacteria...harsh environments...hot springs, salt lakes, soils and marshlands
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archaea are particularly numerous in the | oceans
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there are no known | pathogens in archaea
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bacteria (which is a...) do include... | domain...pathogens
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eukaryotes (which is a...) have a...with... | domain...true nucleus...nuclear membrane
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eukaryotes are things like...which can include... | humans, spiders, flies, plants, molds, yeasts and parasites...pathogens
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bacteria and archaea evolved...and are... | 3-4 billion years ago...unicellular
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eukaryote: protista evolved...and are... | 1.5 bil years ago...unicellular
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eukaryote: fungi evolved...and are... | 1 bil years ago...multi or unicellular
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eukaryote: animalia evolved...and are... | 700 mil years ago...multicellular
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eukaryote: plantae evolved...and are... | 500 mil years ago...multicellular
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ribosomes do...and are they present or not in pro/eu? | protein synthesis...present in both
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cytoskeleton creates the...and provides... | shape in prokaryotes...support, cytoplasmic streaming and endocytosis in eukaryotes
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cytoskeleton is present in some...and in all... | prokaryotes...eukaryotes
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centrioles appear to play a role in...and...and... | mitosis...cytokinesis...flagella and cilia formation in animal cells
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centrioles are...in prokaryotes and... | absent in all....present in animal eukaryots
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membranous organelles are | absent in all prokaryotes
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nucleus is the...of the cell and is...in eukaryotes | control center...present in all
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ER does...and is...in eukaryotes | transportation and lipid synthesis...present in all
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golgi bodies do...and are... eukaryotes | exocytosis and secretion...present in some
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lysosomes do two things...and are...eukaryotes | breakdown of nutrients, self-destruction of damaged or aged cells...present in some
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peroxisomes do...and are...eukaryotes | neutralization of toxins...present in some
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vacuoles do...and are...eukaryotes | storage...present in some
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vesicles do...and are... | transport, digestion and storage...present in all eukaryotes
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mitochondria do..and are... | aerobic ATP production...present in most eukaryotes
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chloroplasts do...and are present in... | photosynthesis...eukaryotic plants, algae and some protozoa
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prokaryotes have only | one copy of DNA (haploid)
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the most important organelle for differentiating between eukaryote and prokaryotes is the | nucleus
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all eukaryotes have a nucleus except for | rbcs
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eukaryotes have organelles for...but prokaryotes... | specific functions...have no organelles
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components of eukaryotic cells whicch would not be considered organelles include | microtubules/filaments and chromosomes
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microtubules and microfilaments make up the...and perform | cytoskeleton structure...movement by forming pseudopoda
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chromosomes are...and do... | organized...packaging of DNA involving histone proteins
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prokaryotic cells range from...and eukaryotic cells range from... | .2-2micrometers...10-100
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prokaryotic flagella consist of | two protein building blocks
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eukaryotic flagella are...and consist of.. | complex...multiple microtubules
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prokaryotic glycocalyx are...and... | present...are a capsule or slime layer
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eukaryotic glycocalx is..that lack... | present in some cells...a cell wall
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prokaryotic plasma membrane contains no..and generally lacks... | carbohydrates...sterols
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the plasma membrane of a eukaryotic cell has...present | sterols and carbohydrates that serve as receptors
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prokaryotic cytoplasm has | no cytoskeleton or cytoplasmic steraming
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eukaryotic cytoplasm has | cytoskeleton and cytoplasmic streaming
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ribosomes of prokaryotic cells are | smaller in size (70s) compared to eukaryotic (80s)
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prokaryotes go through...for cell division | binary fission
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eukaryotic cells go through...for cell division | mitosis
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prokaryotes have | single circular chromosome that lacks histones
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eukaryotes have | multip[le linear chromosomes, with histone arrangement
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prokaryotes and sexual reproduction | no meiosis, transfer of DNA fragments only
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eukaryotes and sexual reproduction | involves meiosis
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cilia are only in...and the motion is in... | eukaryotes...one plane(beating
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flagella are in...but they have... | both pro and eu...different structures
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flagella motion is...or... | rotational in pro...undulation in eukaryotes
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flagella are used for...and direction is determined by... | locomotion...phototaxis or chemotaxis
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phototaxis means | moving toward light
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chemotaxis means | moving toward chemical attractant
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eukaryotic flagella are | internal, entirely surrounded by cytoplasmic membrane
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eukaryotic flagella are only found on...and their action is.. | one pole of cell...rhythmic undulation which pushes or pulls the cell
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prokaryotic flagella are...and several... | imbedded in cell envelope...configurations existt
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prokaryotic flagella action is | rotational, cells move by "run and tumble"
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a run is when...and tumble is when.. | flagella propel prokaryote in a CCW direction..it spins in a CW direction
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prokaryotic flagella have 4 configurations | monotrichous, lophotrichous (polar), amphitrichous (bipolar) and peritrichous
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eukaryotic and prokaryotic flagella function... | differently in their mechanism of propulsion
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eukaryotic flagella | whip back and forth (undulate)
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prokaryotic flagella...and involves the process of... | rotate...chemotaxis
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flagellin contaqin many copies of | a single protein that make up the filament
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flagellin also contain a...that is a.. | basal body...protein
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the prokaryotic flagellin penetrates through the...where it is anchored by the... | cell wall and plasma membrane...basal boedy
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just outside the cell is the...and then the... | hook...filament (made of protein that is specific to that type of cell so you can stereotype them)
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bacterial spirochetes have | axial fibrils in a sheath called axial filaments
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bacterial spirochetes are | helical/spiral-shaped bacteria
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bacterial spirochetes move by...which allows it to... | corkscrewing..burrow into cells
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eukaryotic flagella and cilia do not...and their action is ina... | pierce the cell membrane...beating fashion (not rotary as in prokaryotes)
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cilia are...present only on... | short hair like structures...eukaryotes
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cilia extend from | cell and provide locomotion
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functions of cilia include | locomotion in unicellular organisms and prevention of lung damage in resp tract by pushing out microbes/debris
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motile wbcs move by use of... | pseudopoda
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wbcs are motile but they..by means of the... | crawl...microfilaments and microtubules forming the pseudopods
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pseudopods help perform | phagocytosis
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the ribosomes are a | key difference between pro and eukaryotes (think size)
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70s vs 80s: s is the | sedimentation factor (70 for pro, 80 for euk)
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ribosomal RNA and many proteins differ in | structure and between eukaryotes and prokaryotes
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differing ribosomal RNA is very imp when it comes to | treatment of bacterial diseases by targeting something that is different from oour cells
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prokaryotic cell wall is...and... | usually present...chemically complex (typical bacterial cell wall includes peptidoglycan)
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eukaryotic cell wall, when present is... | chemically simple
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cytosol and inclusions are present in | all pro and euk
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endospores are present in...and... | some pros and absent in all euks
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bacterial morphology/shapes include | bacillus (oval), coccus (round), and spirillum (spiral)
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bacillus example | bacillus anthracis (anthrax)
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coccus example | staphylococcus aureus or MRSA infection
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spirochete example | treponema pallidum or syphillis
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aggregations of cocci can either be | streptococci (chain), diplococci (pair) or staphylococci (clump)
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streptococci example | streptococcus pyrogenes
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diplococci (stop at one cell division) example | neisseria gonorrhea
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staphylococci example | staphylococcus aureus
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cytosol is also called...and is... | cytoplasmic matrix...liquid found inside cells which is complex mixture of substances dissolved in water
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in prokayrotes, most...take place in the cytosol | chemical reactions of metabolism
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cell membrane/cytoplasmic membrane/plasma membrane: like all biological membranes, the bacterial cytoplasmic membrane is composed of | phospholipid and protein molecules
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the phospholipid has a...and a... | hydrophobic part...hydrophilic part
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prokaryotic membranes | lack sterols
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bacterial cytoplasmic membrane does | diffusion, active transport, energy production and motility
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diffusion can either be | passive or facilitated diffsuion through a channel protein
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energy production involves the | electron transport system for bacteria with aerobic and anaerobic respiration (mitochondria)
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bacterial cytoplasmic membrane does motility and involves | the bases of bacterial flagella residing within cell membrane
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diffusion happens through the | phospholipid bilayer
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facilitated diffusion happens through a | nonspecific channel protein
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facilitated diffusion through a...leads to...which leads to... | permease specific for one chemical...binding of substrate...shape change in channel protein
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osmosis is the...through a.. | diffusion of water...specific channel protein or through the phospholipid bilayer
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example of active transport would be | generation of ap roton gradient across membrane for energy production
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active transport uses...and the same principle can be used to pump... | atp...antibiotic drugs out in some mechanisms of drug resistance
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2 major categories based on gram stain | gram positive or negative depending on structure of cell wall
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pili are also called...and they do not do... | fimbriae...movement
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pili are | hair like structures protruding from the cell envelope
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type 1 fimbriae aare...and are used to...which is a... | fuzzy..attach to target tissues...virulence factor
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virulence factors allow | organism to colonize and become pathogenic
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type 2 fimbriae are also called...and are present in... | sex pilus...only gram negative bacteria
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type 2 fimbriae are used for | genetic exchange
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type 2 fimbriae inject | plasmin w/ extra dna to new bacterium
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a capsule is a | discrete mucoid layer of polysaccharides deposited outside the cell wall of bacteria
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sometimes bacterial cells are embedded more...in a...called a.. | randomly...polysaccharide matrix...slime layer or biofilm
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capsules have several functions including | mediating adherence of cells to surfaces (biofilm - dental plaque)and they protect bacterial cells from engulfment by white blood cells (phagocytosis)
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many pathogenic bacteria are | encapsulated in the slime layer which is a virulence factor
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an example of biofilm construction in nature is the formation of...mediated by the... | dental plaque...oral bacterium streptococcus mutans
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bacteria adhere specifically to the... | pellicle of the tooth by means of a protein on the cell surface
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the bacteria are able to...into.. | cleave sucrose (provided by diet)...glucose plus fructose
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fructose is...as an... | fermented...energy source for bacterial growth
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glucose is...into an...that binds the... | polymerized..extracellular dextran polymer...bacteria to the enamel and forms a biofilm some 300-500 cells in thickness
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biofilm cements the | bacteria to the tooth enamel and becomes the matrix of dental plaque
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the dextran slime can be...for use as a... | depolymerized to glucose...carbon source
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depolymeriing dextran slime results in production of..within the biofilm that... | lactic acid...decalcifies the enamel and leads to dental caries or bacterial infection of the tooth
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