Transfer of Bacteria: Aseptic Technique
Quiz yourself by thinking what should be in
each of the black spaces below before clicking
on it to display the answer.
Help!
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| Why flame the loop? | To incinerate any organisms on the loop
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| Why flame the mouth of the tube? | Because it'll kill microorganisms on the tube and convection currents will prevent airborne contaminants from entering the tube.
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| Why wait for the loop to cool? | To prevent killing of bacteria to be transfered and to prevent formation of aerosols.
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| What other methods can be used to determine motility aside from wet mount? | hanging-drop procedure
flagella stain &
semisolid stab culture.
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| What is the primary use of slants? | To store pure cultures
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| What is the primary use of deeps? | To store anaerobic cultures or to test oxygen requirements
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| What is the primary use of broths? | To obtain a large number of bacteria
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| What is the purpose of flaming the loop before use? | To prevent contamination of an experiment by unwanted organisms.
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| What is the purpose of flaming the loop after use? | To prevent contamination of the laboratory worker by organisms being studied
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| Why must the loop be cool before you touch it to a culture? | To avoid killing desired organisms
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| Should you set the loop down to let it cool? Why or why not? | No. To avoid contamination by microorganisms on the workbench
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| How do you determine when the loop is cool? | Through trial and error
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