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Micro-Lab1
Transfer of Bacteria: Aseptic Technique
| Question | Answer |
|---|---|
| Why flame the loop? | To incinerate any organisms on the loop |
| Why flame the mouth of the tube? | Because it'll kill microorganisms on the tube and convection currents will prevent airborne contaminants from entering the tube. |
| Why wait for the loop to cool? | To prevent killing of bacteria to be transfered and to prevent formation of aerosols. |
| What other methods can be used to determine motility aside from wet mount? | hanging-drop procedure flagella stain & semisolid stab culture. |
| What is the primary use of slants? | To store pure cultures |
| What is the primary use of deeps? | To store anaerobic cultures or to test oxygen requirements |
| What is the primary use of broths? | To obtain a large number of bacteria |
| What is the purpose of flaming the loop before use? | To prevent contamination of an experiment by unwanted organisms. |
| What is the purpose of flaming the loop after use? | To prevent contamination of the laboratory worker by organisms being studied |
| Why must the loop be cool before you touch it to a culture? | To avoid killing desired organisms |
| Should you set the loop down to let it cool? Why or why not? | No. To avoid contamination by microorganisms on the workbench |
| How do you determine when the loop is cool? | Through trial and error |
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